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Secondary structure of T2 intraphage DNA: a spectrophotometric study

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Il Nuovo Cimento D

Summary

Relevant differences in the spectral properties (in the (230÷320) nm range) between intraphage and free T2 DNA are reported in this work. These differences are very similar to the variation in absorption obtained for a native DNA solution when the pH is lowered starting from the neutral value. On this basis, cytosine protonation in intraphage DNA is hypothesized.

Riassunto

In questo lavoro si mettono in evidenza significative differenze nelle proprietà spettrali del DNA di T2 intrafagico e libero in soluzione nell'intervallo (230÷320) nm. Queste differenze sono molto simili a quelle ottenute in una soluzione di DNA nativo abbassando il pH a partire dal valore neutro. Su questa base si avanza l'ipotesi di una protonazione della citosina nel DNA intrafagico.

Резюме

В этой работе рассматриваются различия в спектральных свойствах (в области (230÷320) нм) между внутрифаговыми и свободными T2 ДНК. Эти различия очень сходны с изменениями в поглощении, полученными для естественного раствора ДНК, когда pH уменьшается по сравнению с нейтральной величиной. На основе этого подхода выдвигается гипотеза протонации цитозина во внутрифаговой ДНК.

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Authors and Affiliations

  1. Laboratorio di Fisica, Istituto Superiore di Sanità, Roma

    M. Matzeu & O. M. Quaranta

  2. Istituto di Fisica dell'Università, Perugia

    G. Onori

Authors
  1. M. Matzeu
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  2. O. M. Quaranta
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  3. G. Onori
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Matzeu, M., Quaranta, O.M. & Onori, G. Secondary structure of T2 intraphage DNA: a spectrophotometric study. Il Nuovo Cimento D 2, 1480–1488 (1983). https://doi.org/10.1007/BF02460226

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  • DOI: https://doi.org/10.1007/BF02460226

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