Skip to main content
SpringerLink
Log in

Structural variations induced by magnesium ions and temperature in the large ribosomal subunit from the extreme thermophile Sulfolobus solfataricus

  • Published:
Il Nuovo Cimento D

Summary

Small-angle neutron scattering (SANS) measurements on the large 50S ribosomal subunit of the extreme thermophile archaebacterium Sulfolobus solfataricus are presented. The structural effects induced by magnesium ions and temperature are investigated. Supramolecular aggregation was present in all the investigated conditions. Removal of magnesium ions and increase of temperature reduce the intensity of the scattering at Q=0. This reduction is discussed looking at the Fourier inversion of the scattering intensity, which includes both inter- and intra-particle contributions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. W. E. Hill, A. Dahlberg, R. A. Garrett, P. B. Moore, D. Schlessinger and J. W. Warner (Editors): The Ribosome. Structure, Function and Evolution (American Society for Microbiology, Washington, D.C., 1990).

    Google Scholar 

  2. P. Londei, S. Altamura, E. Caprini and A. Martayan: Biochimie, 73, 1465 (1991).

    Article  Google Scholar 

  3. P. Londei, J. Teixidò, M. Acca, P. Cammarano and R. Amils: Nucleic Acids Res., 14, 2269 (1986).

    Article  Google Scholar 

  4. H. F. Noller, V. Hoffarth and L. Zimniak: Science, 256, 1416 (1992).

    Article  ADS  Google Scholar 

  5. H. B. Stuhrmann: in Methods of Experimental Physics, Vol. 23, part C (Academic Press, New York, N.Y., 1987).

    Google Scholar 

  6. A. S. Spirin, V. I. Baranov, G. S. Polubesov, I. N. Serdyuk and R. P. May: J. Mol. Biol., 194, 119 (1987).

    Article  Google Scholar 

  7. O. Glatter: J. Appl. Crystallogr., 10, 415 (1977).

    Article  Google Scholar 

  8. P. B. Moore: J. Appl. Crystallogr., 13, 168 (1980).

    Article  Google Scholar 

  9. G. Briganti, A. Giansanti, R. Giordano and F. Pedone: Physica B, in press (1994).

Download references

Author information

Authors and Affiliations

  1. Dipartimento di Fisica, Università degli Studi di Roma «La Sapienza», P.le A. Moro 2, 00185, Roma, Italy

    G. Briganti & A. Giansanti

  2. Dipartimento di Fisica dell’Università, Salita Sperone, 98100, S. Agata (Me), Italy

    R. Giordano

  3. Dipartimento di Biopatologia Umana, Sezione Biologia Cellulare, Università degli Studi di Roma «La Sapienza», Viale Regina Elena 324, 00185, Roma, Italy

    P. Londei

  4. Dipartimento di Genetica e Biologia Molecolare, Università degli Studi di Roma «La Sapienza», P.le A. Moro 2, 00185, Roma, Italy

    F. Pedone

Authors
  1. G. Briganti
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Giansanti
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Giordano
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. P. Londei
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. F. Pedone
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Briganti, G., Giansanti, A., Giordano, R. et al. Structural variations induced by magnesium ions and temperature in the large ribosomal subunit from the extreme thermophile Sulfolobus solfataricus . Nouv Cim D 16, 1573–1577 (1994). https://doi.org/10.1007/BF02462044

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02462044

PACS 87.15.Kg

PACS 01.30.Cc

Search

Navigation