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Preparative isolation ofSolanum tuberosum L. glycoalkaloids by MPLC

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Summary

A new, efficient and economic method employing Medium Pressure Liquid Chromatography (MPLC) for the isolation of the two majorSolanum tuberosum L. glycoalkaloids (α-solanine and α-chaconine) is described. Potato peelings are homogenised with 5% acetic acid, the glycoalkaloids purified by filtration through an XAD-2 column and then by precipitation from the aqueous solution. The resulting glycoalkaloid fraction was purified by MPLC using a Silica Gel column and a CHCl3:MeOH:2% NH4OH mixture (70∶30∶5) as mobile phase to yield pure α-chaconine and a-solanine. This methodology can be used to obtain glycoalkaloids for enthomology and toxicological research where large amounts of these compounds are required.

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Authors and Affiliations

  1. Facultad de Química, Cátedra de Farmacognosia y Productos Naturales, Avda. General Flores 2124, C.C. 1157, Montevideo, Uruguay

    S. Soulé, A. Vázquez, G. González, P. Moyna & F. Ferreira

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  1. S. Soulé
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  2. A. Vázquez
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  3. G. González
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  4. P. Moyna
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  5. F. Ferreira
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Soulé, S., Vázquez, A., González, G. et al. Preparative isolation ofSolanum tuberosum L. glycoalkaloids by MPLC. Potato Res 40, 413–416 (1997). https://doi.org/10.1007/BF02358001

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  • DOI: https://doi.org/10.1007/BF02358001

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