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Discrete intracellular Ca2+ pools coupled to two distinct Ca2+ influx pathways in human platelets

  • Original Paper
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Journal of Biomedical Science

Abstract

Ca2+ influx is an important event associated with platelet activation and regulated by the content of intracellular Ca2+. Previous studies have suggested two different Ca2+ pools and two Ca2+ influx pathways exist in platelets. In the present study, we have investigated the regulation of thrombin- and thapsigargin-induced Ca2+ entry into human platelets, using fluorescent indicators to monitor Ca2+ mobilization and membrane potential. It was found that depletion of thapsigargin-sensitive Ca2+ stores was coupled to Ca2+ influx through a Ca2+-selective pathway. Additional release of Ca2+ from the thapsigargin-insensitive pool by thrombin caused the opening of a nonselective cation channel.

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Authors and Affiliations

  1. Vascular Biology, Maryland Research Laboratories, Otsuka America Pharmaceutical, Inc., 9900 Medical Center Drive, 20878, Rockville, MD, USA

    Bing Sun & Jun-ichi Kambayashi

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  1. Bing Sun
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  2. Jun-ichi Kambayashi
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Sun, B., Kambayashi, Ji. Discrete intracellular Ca2+ pools coupled to two distinct Ca2+ influx pathways in human platelets. J Biomed Sci 7, 35–41 (2000). https://doi.org/10.1007/BF02255916

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  • DOI: https://doi.org/10.1007/BF02255916

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