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Expression of TAR RNA-binding protein in baculovirus and co-immunoprecipitation with insect cell protein kinase

  • Original Paper
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Journal of Biomedical Science

Abstract

TAR RNA-binding protein TRBP was originally isolated by its binding affinity for radiolabeled HIV-1 leader RNA. Subsequent studies have suggested that this protein is one member of a family of double-stranded RNA-binding proteins. Recent findings indicate that TRBP might function to antagonize the translational inhibitory effect that can be mediated through cellular protein kinase, PKR. Here, we report on the over-expression of a cDNA coding for TRBP in eukaryotic SF9 cells using baculovirus. We characterized the nuclear localization of TRBP in insect cells, and we demonstrate that TRBP co-immunoprecipitates with a protein in these cells antigenically related to human PKR.

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Authors and Affiliations

  1. The Wellcome Research Laboratories Langley Court, South Eden Park Road, BR3 3BS, Beckenham, Kent, UK

    Edward D. Blair, Christopher M. Roberts & B. Wendy Snowden

  2. Molecular Virology Section, Laboratory of Molecular Microbiology, NIAID, NIH, Bethesda, Md., USA

    Anne Gatignol, Monsef Benkirane & Kuan-Teh Jeang

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  1. Edward D. Blair
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  2. Christopher M. Roberts
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  3. B. Wendy Snowden
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  4. Anne Gatignol
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  5. Monsef Benkirane
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  6. Kuan-Teh Jeang
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Blair, E.D., Roberts, C.M., Snowden, B.W. et al. Expression of TAR RNA-binding protein in baculovirus and co-immunoprecipitation with insect cell protein kinase. J Biomed Sci 2, 322–329 (1995). https://doi.org/10.1007/BF02255219

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  • DOI: https://doi.org/10.1007/BF02255219

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