Skip to main content
SpringerLink
Log in

Production of fructooligosaccharides from sucrose by a transfructosylase from Aspergillus niger

  • Research Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

A strain of Aspergillus niger isolated from sugarcane fields, produced an extracellular transfructosylase in the culture medium. Sucrose and raffinose induced the production to the enzyme, which was purified by 138-fold. The optimum pH for activity and stability were 5.5 and 6.5, respectively. Its optimum temperature was 55°C. The enzyme hydrolysed sucrose rapidly and simultaneously formed fructooligosaccharides by transfructosylation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bealing, F.J. 1953 Mould glucosaccharase: a fructose. Biochemical Journal 55, 93–101.

    Google Scholar 

  • Bealing, F.J. & Bacon, J.S.D. 1953 The action of mould enzymes on sucrose. Biochemical Journal 53, 277–285.

    Google Scholar 

  • Dziezak, J.D. 1986 Special report: Sweeteners and product development. Food Technology 40, 111–128.

    Google Scholar 

  • Gupta, A.K. & Bhatia, T.S. 1980 Glucofructosan biosynthesis in Fusarium oxysporum. Phytochemistry 19, 2557–2563.

    Google Scholar 

  • Hidaka, H., Hirayama, M. & Sumi, N. 1988 A fructooligosaccharide-producing enzyme from Aspergillus niger ATCC 20611. Agricultural and Biological Chemistry 52, 1181–1187.

    Google Scholar 

  • Hirayama, M., Sumi, N. & Hidaka, H. 1989 Purification and properties of a fructooligosaccharide-producing β-fructofuranosidase from Aspergillus niger ATCC 20611. Agricultural and Biological Chemistry 53, 667–673.

    Google Scholar 

  • Jung, K.H., Yun, J.W., Kang, K.R., Lim, J.Y. & Lee, J.H. 1989 Mathematical model for enzymatic production of fructooligosaccharides from sucrose. Enzyme and Microbial Technology 11, 491–494.

    Google Scholar 

  • Pazur, J.H. 1952 Transfructosidation reactions of an enzyme of Aspergillus oryzae. Journal of Biological Chemistry 199, 217–225.

    Google Scholar 

  • Trevelyan, W.E., Procter, D.P. & Harrison, J.G. 1950 Detection of sugars on paper chromatograms. Nature 166, 444–445.

    Google Scholar 

Download references

Authors
  1. Y. K. Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. M. Almeida
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

The authors are with the University of Campinas, College of Food Engineering, Laboratory of Food Biochemistry (UNICAMP), Caixa postal 6121, Campinas, SP, Brazil.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Park, Y.K., Almeida, M.M. Production of fructooligosaccharides from sucrose by a transfructosylase from Aspergillus niger . World Journal of Microbiology and Biotechnology 7, 331–334 (1991). https://doi.org/10.1007/BF00329399

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00329399

Keywords

Search

Navigation