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Effects of incubation temperature on microspore callus production and plant regeneration in wheat anther cultures

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Abstract

Anthers of wheat cultivars Orofen and Pitic 62 were incubated for 8 days at 15, 20, 25, 30, 35 and 40°C before transfer to 25°C. Compared with anthers cultured at 25°C constantly, anthers treated at 30°C produced 40% more microspore callus and green plants in both cultivars whereas those treated at 35°C produced 2–3 fold more green plants. Treatment at 40°C was deleterious. Possible modes of action of high temperature on callus production and albinism were discussed.

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Author notes

  1. Bin Huang

    Present address: Horticulture Department, Purdue University, 47907, West Lafayette, IN, USA

Authors and Affiliations

  1. Institute of Genetics, Academia Sinica, Peking, PRC

    Bin Huang

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  1. Bin Huang
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Huang, B. Effects of incubation temperature on microspore callus production and plant regeneration in wheat anther cultures. Plant Cell Tiss Organ Cult 9, 45–48 (1987). https://doi.org/10.1007/BF00046078

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  • DOI: https://doi.org/10.1007/BF00046078

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