Skip to main content
SpringerLink
Log in

A study on floral biology of seedlings in vitro in Orychophragmus violaceus: Induction of flowers in seedlings of O. violaceus cultured in vitro

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Seedlings of O. violaceus were cultured on MS media and treated at low temperature. Cold treatment at 5–7 °C for more than 7 days was needed for flower induction of seedlings in vitro originated from germinated seeds. When cultured on MS media supplemented with 2.5 mg l−1 zeatin and 2 mg l−1 gibberellin (GA3), seedlings in vitro did initiate flowers without cold treatment. When MS media was used with a reduced amount of NH4NO3, flower induction of seedlings in vitro could be accelerated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Chen YL (1990) A list of plant species with test-tube flowers. Comm. Plant Physiol. 3: 75–76 (in Chinese)

    Google Scholar 

  • Coombs J, Hall DO, Long SP & Scurlock JMO (1985) Techniques in Bioproductivity and Photosynthesis. 2nd edn, Pergamon Press

  • Li WA (1988) Studies on fast plant propagation by means of tissue culture in China. Comm. Plant Physiol. 4: 12 (in Chinese)

    CAS  Google Scholar 

  • Linsmaier EM & Skoog F (1965) Organic growth factor requirements of tobacco tissue culture. Physiol. Plant. 40: 100–127

    Article  Google Scholar 

  • Loo SV (1946) Cultivation of excised stem tips of dodder in vitro. Amer. J. Bot. 33: 395–400

    Google Scholar 

  • Luo P & Huang BQ (1996) A study on exploitation of new vegetable germplasm Orychopragmus violaceus OE Schulz, a member of Brassicaceae. Acta Hortic. 407: 75–78

    CAS  Google Scholar 

  • Luo P, Huang BQ, Yin JM, Chen ZL, Chen YH & Lan ZQ (1998) A new forage genetic resource Orychophragmus violaceus OE Schulz. Gen Res Crop Evol. 45: 491–494

    Article  Google Scholar 

  • Luo P, Lan ZQ & Li ZY (1994) Orychophragmus violaceus, a potential edible oil crop. Plant Breeding 113: 83–85

    Article  Google Scholar 

  • Luo P, Zhou JM, Wu YY & Zhang XM (1996) Experimental results of the regeneration ability of different explants of Orychophragmus violaceus. Hereditas (Beijing) 18(1): 23–25 (in Chinese)

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bloassays with plant tissue culture. Physiol. Plant. 15: 473–497

    Article  CAS  Google Scholar 

  • Simmonds J (1982) In vitro flowering of leaf explants of Streptocarpus nobilis: The influence of culture medium components on vegetative and reproductive development. Can. J. Bot. 60: 1461–1468

    Google Scholar 

  • Tanimoto S & Harada H (1981) Effect of IAA, zeatin, ammonium nitrate and sucrose on the initiation and development of flower buds in Torenia. Plant Cell Physiol. 22: 1553–1560

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Botany, Sichuan University, Chengdu, 610064, China

    Peng Luo & Ze-qu Lan

  2. Yibin Normal College, Yibin, Sichuan, 644007, China

    Qiu Ye

Authors
  1. Peng Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qiu Ye
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ze-qu Lan
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Luo, P., Ye, Q. & Lan, Zq. A study on floral biology of seedlings in vitro in Orychophragmus violaceus: Induction of flowers in seedlings of O. violaceus cultured in vitro . Plant Cell, Tissue and Organ Culture 63, 73–75 (2000). https://doi.org/10.1023/A:1006262227785

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1006262227785

Search

Navigation