Abstract
This work presents the preliminary results of in vitro studies with Araujia sericifera, which is cultivated for ornamental purposes. Immature seeds from wild plants were used to start the cultures. Somatic embryos and friable embryogenic calluses were obtained from white cotyledons in media containing naphthaleneacetic acid and benzyladenine or 2,4-dichlorophenoxyacetic acid. Plants were regenerated from these somatic embryos.
Cell suspensions obtained from friable calluses cultured in M1 modified medium showed a considerable growth capacity. The packed cell volume was doubled in about 15 days of culture at the exponential phase. the results obtained may be used to design further experiments with the aim of improving somatic embryogenesis.
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Abbreviations
- NAA:
-
α-naphthaleneacetic acid
- BA:
-
benzyladenine
- IBA:
-
indolebutyric acid
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
References
Armstrong CL & Green CE (1985) Establishment and maintenance of friable embryogenic maize callus and the involvement of L-proline. Planta 164: 207–214
Atanassov AI (1986) Sugar beet. In: Evans DA, Sharp WR & Ammirato PV (Eds) Handbook of Plant Cell Culture, Vol 4 (pp 602–680). Macmillan, New York
Bajaj YPS & Nietsch P (1975) In vitro propagation of red cabbage (Brassica oleracea L. var. capitata). J. Exp. Bot. 26: 883–890
Bhatia CR, Murty GSS & Mathews VH (1985) Regeneration of plants from ‘de-embryonated’ peanut cotyledons cultured without nutrients and agar. Z. Pflanzenzücht. 94: 149–155
Harbaoui Y & Debergh P (1980) Multiplication in vitro de clones selectionnes d'artichaut (Cynara scolymus L.). In: Doré C (Ed) Application de la Culture In Vitro à l'Amélioration des Plantes Potagères (pp 1–7). Eucarpia meeting, Versailles
Murashige T & Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Nitsch JP & Nitsch C (1969) Haploid plants from pollen grains. Science 163: 85–87
Novak FJ, Domini D & Owusu G (1986) Somatic embryogenesis and in vitro plant development of cacao (Theobroma cacao). In: Proceedings of Symposium on Nuclear Techniques and In Vitro Culture for Plant Improvement (pp 443–449). IAEA, Vienna
Pecaut P, Dumas de Vaulx R & Lot H (1983) Virus-free clones of globe artichoke (Cynara scolymus) obtained after in vitro propagation. Acta Hort 131: 303–309
Torné JM, Santos MA, Pons A & Blanco M (1980) Regeneration of plants from mesocotyl tissue cultures of immature embryos of Zea mays L. Plant Sci. Lett. 17: 339–344
Torné JM, Santos MA & Blanco JL (1984) Methods of obtaining maize totipotent tissues: II-Atrophic tissue culture. Plant Sci. Lett 33: 317–325
Webb DT, Torres LD & Fobert P (1984) Interactions of growth regulators, explant age and culture environment controlling organogenesis from lettuce cotyledons in vitro. Can. J. Bot. 62: 586–590
Zimmerman TW & Ascher PD (1982) Tissue culture of the lily embryo cotyledon. HortScience 17: 104 (Abstract)
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Torné, J.M., Claparols, I. & Santos, M.A. Somatic embryogenesis in Araujia sericifera . Plant Cell Tiss Organ Cult 29, 269–274 (1992). https://doi.org/10.1007/BF00034362
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DOI: https://doi.org/10.1007/BF00034362