Abstract
The phytotoxic effects of antibiotic treatment on micropropagated Clematis, Delphinium, Hosta, Iris and Photinia were determined by assessing multiplication and rooting rates of plants in vitro and weaning success and flowering in vivo.
Similar content being viewed by others
Abbreviations
- BA:
-
6-benzyladenine
- IAA:
-
3-indole-acetic acid
- NAA:
-
1-naphthyl-acetic acid
References
Bastiaens L, Maene L, Harbaoui Y, van Sumere C, van de Castelle KL & Debergh PC (1983) The influence of antibacterial products on plant tissue cultures. Meded Faculteit Landbouwwettenschappen Rijksuniversiteit, Gent 48: 13–24
Brian PW (1957) Effects of antibiotics on plants. Annu Rev. Plant Pathol. 8: 413–426
Cornu D & Michel MF (1987) Bacteria contaminants in shoot cultures of Prunus avium L. choice and phytotoxicity of antibiotics. Acta Hort. 212: 83–86
Dodds JH & Roberts LW (1981) Some inhibitory effects of gentamicin on plant tissue cultures. In Vitro 17: 467–470
Falkiner FR (1990) The criteria for choosing an antibiotic for control of bacteria in plant tissue culture. Intl. Assoc. Plant Tiss. Cult. Newsl. 60: 14–21
Fisse J, Batalle A & Pera J (1987) Endogenous bacteria elimination in ornamental plants. Acat Hort. 212: 87–90
Garrod LP, Lambert HP & O'Grady F (1973) Antibiotic and Chemotherapy (pp 301–306). Churchill Livingstone, Edinburgh/London
Horsch RB & King J (1983) A covert contaminant of cultured plant cells: elimination of a Hyphomicrobium spp. from cultures of Datura innoxia (Mill.). Plant Cell Tiss. Org. Cult. 2: 21–28
Klastersky J (1975) Clinical Use of Combinations of Antibiotics. Hodder & Stoughton, London/Sydney
Leggatt I, Waites WM, Leifert C & Nicholas JR (1988) Characterisation of micro-organisms isolated from plants during micropropagation. Acta Hort. 225: 93–102
Leifert C (1990) Contaminants of plant tissue cultures. Ph.D. thesis Nottingham University, Faculty of Agricultural and Food Science
Leifert C, Waites WM & Nicholas JR (1989a) Bacterial contaminants of micropropagated plant cultures. J. Appl. Bacteriol. 67: 353–361
Leifert C, Waites WM, Camotta H & Nicholas JR (1989b) Lactobacillus plantarum; a deleterious contaminant of plant tissue culture. J. Appl. Bacteriol. 67: 363–370
Leifert C, Ritchie JY & Waites WM (1991a) Contaminants of plant tissue and cell cultures. World J. Microbiol. Biotechnol. 7: 452–469
Leifert C, Camotta H, Wright SM, Waites B, Cheyne VA & Waites WM (1991b) Elimination of bacteria from micropropagated plant cultures using antibiotics. J. Appl. Bacteriol. 71: 307–330
Long RD, Curtin TF & Cassells AC (1988) An investigation of the effects of bacterial contaminants on potato nodal cultures. Acta Hort. 225: 83–91
McCown BH & Lloyd GB (1983) A survey of the response of Rhododendron to in vitro culture. Plant Cell Tiss. Org. Cult. 2: 75–85
Mathias PJ, Alderson PG & Leakey RRB (1987) Bacterial contamination in tropical hardwood cultures. Acta Hort. 212: 43–49
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Owens LD (1979) Kanamycin promotes morphogenesis of plant tissues. Plant Sci. Lett. 16: 225–230
Phillips R, Arnott SN & Kaptan SE (1981) Antibiotics in plant tissue culture: Rifampicin effectively controls bacterial contaminants without affecting the growth of short-term explant cultures of Helianthus tuberousus. Plant Sci. Lett. 21: 235–240
Podwyzynska M & Hempel M (1987) Identification and elimination of ‘slowly growing’ bacteria from a micropropagated Gerbera. Acta Hort. 212: 112
Pollock K, Barfield DG & Shields R (1983) The toxicity of antibiotics to plant cell cultures. Plant Cell Rep. 2: 36–39
Poulsen GB (1988) Elimination of contaminating microorganisms from meristem culture of apple rootstock M26. Acta Hort. 225: 193–197
Santos I & Salema R (1989) Penicillins and activity of nitrogen metabolism enzymes in plant tissue culture. Plant Sci. 59: 119–125
Thurston KC, Spencer SJ & Arditti J (1979) Phytotoxicity of fungicides and bactericides in orchid culture media. Amer. J. Bot. 66: 825–835
Young PM, Hutchins AS & Canfield ML (1984) Use of antibiotics to control bacteria in shoot cultures of woody plants. Plant Sci Lett. 34: 203–209
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Leifert, C., Camotta, H. & Waites, W.M. Effect of combinations of antibiotics on micropropagated Clematis, Delphinium, Hosta, Iris and Photinia . Plant Cell Tiss Organ Cult 29, 153–160 (1992). https://doi.org/10.1007/BF00033621
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00033621