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Callus regeneration from Trifolium subterraneum protoplasts and enhanced protoplast division by low-voltage treatment and nurse cells

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Abstract

Seedling and suspension culture protoplasts of subterranean clover (Trifolium subterraneum L.) were successfully cultured in semi-solid drops of calcium alginate and ultrafiltered liquid medium. Protoplast-derived subterranean-clover colonies developed as the osmolality was lowered over three steps. Callus was established from these colonies. Calli derived from protoplasts have failed to regenerate on a range of media. The frequency of dividing subterranean-clover protoplasts was increased in the presence of lucerne (Medicago sativa L.) nurse cells. Low-voltage treatments (200 mV) for the first 16–132 hours of culture also resulted in a 100% increase in the frequency of dividing protoplasts.

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  1. Li Zhongyi

    Present address: Department of Biology, Shandong University, Peoples' Republic of China

Authors and Affiliations

  1. CSIRO Division of Plant Industry, P.O. Box 1600, 2601, Canberra, Australia

    Li Zhongyi, G. J. Tanner & P. J. Larkin

Authors
  1. Li Zhongyi
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  2. G. J. Tanner
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  3. P. J. Larkin
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Zhongyi, L., Tanner, G.J. & Larkin, P.J. Callus regeneration from Trifolium subterraneum protoplasts and enhanced protoplast division by low-voltage treatment and nurse cells. Plant Cell Tiss Organ Cult 21, 67–73 (1990). https://doi.org/10.1007/BF00034494

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  • DOI: https://doi.org/10.1007/BF00034494

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