Skip to main content
Log in

Effects of fungicides and bactericides on orchid seed germination and shoot tip cultures in vitro

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Amphotericin B, benomyl, gentamycin, nystatin, quintozene penicillin G, sodium omadine, and vancomycin singly and in several combinations have no deleterious effects on the germination of orchid seeds, but inhibit the growth in vitro of shoot tip explants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Al-Beldawi AS, Pinckard JA (1970) Control ofRhizoctonia solani on cotton seedlings by means of Benomyl. Plant Dis Rep 54:76–80

    Google Scholar 

  2. Arditti J (1977) Clonal propagation of orchids by means of tissue culture — a manual. In: Arditti J (ed) Orchid biology, reviews and perspectives, I. Ithaca: Cornell University Press, pp 203–293

    Google Scholar 

  3. Arditti J (1979) Origin of protocorm. Am Orchid Soc Bull 48:228

    Google Scholar 

  4. Carew DP, Patterson BD (1970) The effect of antibiotics on the growth ofCatharanthus roseus tissue cultures. Lloydia 33:275–277

    CAS  PubMed  Google Scholar 

  5. Byford WJ, Prince J (1976) Experiments with fungicides to controlAphanomyces cocliodes in sugar beet. Ann Appl Biol 83:69–77

    CAS  Google Scholar 

  6. Davis RD, Pinckard JA (1971) Comparative systemic fungicidal activity and phytotoxicity of certain seed and soil fungicides potentially useful for control of cotton seedling diseases. Plant Dis Rep 55:1111–1115

    CAS  Google Scholar 

  7. Dressler RL (1981) The orchids, natural history and classification. Cambridge: Harvard University Press

    Google Scholar 

  8. El-Zayyat MM, Sawhney IS, Ablack M, Nashnoosh I (1974) Effect of some seed dressings on germination of wheat grain. Libyan J Agric 3:85–88

    CAS  Google Scholar 

  9. Griffiths DA (1965) Temporary control of intertestal contamination during growth of seedlings on nutrient agar. Can J Microbiol 11:1025–1027

    CAS  PubMed  Google Scholar 

  10. Hamawi H, Mahdi MT, El Ghandour MA, Eid A, Sheltawi E (1977) Effect of seed treatment with pesticides on germination of some Egyptian cotton cultivars. Seed Sci Technol 5:97–103

    CAS  Google Scholar 

  11. Harrison C (1970) A simple method for flasking orchid seeds. Am Orchid Soc Bull 39:715–716

    Google Scholar 

  12. Mariat F (1952) Recherches sur la physiologie des embryons des orchidées. Rev Gen Bot 59:324–377

    Google Scholar 

  13. Maude RB, Kyle AM (1970) Seed treatment with benomyl and other fungicides for the control ofAscochyta pisi on peas. Ann Appl Biol 66:37–41

    CAS  Google Scholar 

  14. Mills JT, Wallace HAH (1972) Differential action of fungicides upon fungi occurring on wheat, barley, buckwheat, and oil seeds. Can J Plant Sci 52:281–290

    CAS  Google Scholar 

  15. Owens LD (1979) Kanamycin promotes morphogenesis of plant tissues. Plant Sci Lett 16:225–230

    CAS  Google Scholar 

  16. Paul B, Thapar VK, Singh R (1973) Effect of systemic fungitoxicants on ureide metabolism in germinating seeds. Indian J Agric Sci 43:662–666

    CAS  Google Scholar 

  17. Phillips R, Arnott SM, Kaplan SE (1981) Antibiotics in plant tissue culture: rifampicin effectively controls bacterial contaminants without affecting the growth of short-term explant cultures ofHelanthus tuberosus. Plant Sci Lett 21:235–240

    CAS  Google Scholar 

  18. Skene KGM (1972) Cytokinin-like properties of the systemic fungicide benomyl. J Hortic Sci 47:179–182

    CAS  Google Scholar 

  19. Solel Z, Schooley JM, Edgington LV (1973) Uptake and translocation of benomyl and carbendazim (methyl benzimidazol-2-yl carbamate) in the symplast. Pestic Sci 4:713–718

    CAS  Google Scholar 

  20. Spencer SJ, Thurston KC, Johnson JA, Perera RG, Arditti J (1979-1980) Media which do not require sterilization for the culture of orchid seedlings and Phalaenopsis flower stalk nodes. Malay Orchid Rev 14:44–53

    Google Scholar 

  21. Spoerl E (1948) Amino acids as sources of nitrogen for orchid embryos. Am J Bot 35:88–95

    CAS  Google Scholar 

  22. Thomas TH (1973) Growth regulatory effects of three benzimidazole fungicides on the germination of celery (Apium graveolens) seeds. Ann Appl Biol 74:233–238

    CAS  Google Scholar 

  23. Thurston KC, Spencer SJ, Arditti J (1979) Phytotoxicity of bactericides and fungicides in orchid culture media. Am J Bot 66:825–835

    CAS  Google Scholar 

  24. Thurston KC, Spencer SJ, Arditti J (1980) Incorporation of fungicides and bactericides in orchid seed and seedling culture media. Proc 9th World Orchid Conf, Bangkok, 1978, pp 179–190

  25. Tsuchiya J (1954) Possibility of germination of orchid seeds from immature fruits. Na Pua Okika O Hawaii Nei-Orchids Hawaii 4:11–16

    Google Scholar 

  26. Williams PF (1975) Cytokinin activity of benomyl. Aust Plant Pathol Soc Newslett 4:12–13

    Google Scholar 

  27. Wimber DE (1963) Clonal multiplication of cymbidiums through tissue culture of the shoot meristem. Am Orchid Soc Bull 32:105–107

    Google Scholar 

  28. Yang H-J (1976) Effect of benomyl onAsparagus officinalis L. shoot and root development in culture media. HortScience 11:473–474

    CAS  Google Scholar 

  29. Zutshi U, Kaul BL (1975) Studies on the cytogenetic activity of some common fungicides in higher plants. Cytobios 12:61–67

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Brown, D.M., Groom, C.L., Cvitanik, M. et al. Effects of fungicides and bactericides on orchid seed germination and shoot tip cultures in vitro. Plant Cell Tiss Organ Cult 1, 165–180 (1981). https://doi.org/10.1007/BF02318914

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02318914

Key words

Navigation