Abstract
Photosynthetic parameters of the lichen Ramalina duriael were investigated in vivo, by the photoacoustic method using intensity—modulated exciting light (frequency range 5–300 Hz). The photoacoustic signal in intact lichens was similar in its general characteristics to that obtained from intact leaves of higher plants (Poulet et al., Biochim. Biophys. Acta 724, 433–446, 1983). It included two components interpreted to be due to modulated heat and modulated oxygen evolution. The quantum yield of the oxygen evolution component was maximal in the red spectral region and exhibited the ‘red drop’ decrease at wavelengths larger than 680 nm, similar to observations in higher plants. In contrast to those however, there was a pronounced decrease in this yield in the region below about 600 nm, indicating that pigments absorbing at shorter wavelengths are inefficient energy transfer agents. Similar results were observed for the quantum yield spectrum of photochemical energy storage. Analysis of oxygen diffusion in the symbiont alga, from the modulation frequency dependence of the ratio of oxygen evolution to photothermal signal component is consistent with an average diffusion path of about 4 μm, compared to a smaller, 1 μm, average diffusion path obtained in green leaves.
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Bults G, Horwitz PA, Malkin S and Cahen D (1982) Photoacoustic measurements of photosynthetic activities in whole leaves—photochemistry and gas exchange. Biochim Biophys Acta 679, 452–465
Poulet P, Cahen D and Malkin S (1983) Photoacoustic detection of photosynthetic oxygen evolution from leaves—quantitative analysis by phase and amplitude measurements. Biochim Biophys Acta 724, 433–446
Canaani O, Cahen D and Malkin S (1982) Photosynthetic chromatic transitions and Emerson enhancement effects in intact leaves studied by photoacoustics. FEBS Lett 150, 142–146
Canaani O and Malkin S (1984) Distribution of light excitation in an intact leaf between the two photosystems of photosynthesis—changes in absorption cross-sections following state 1—state 2 transitions. Biochim Biophys Acta, in press
Canaani O and Malkin S (1984) Physiological adaptation to a new low-light intensity state in intact leaves resulting in an extreme imbalance in excitation energy distribution between the two photosystems. Biochim Biophys Acta, in press
Charpentier P, Lepoutre F and Bertrand LJ (1982) Photoacoustic measurements of thermal diffusivity: description of the ‘Drum Effect’. Appl Phys 53, 608–614
Strutt JW (Lord Rayleigh) The Photophore (1981) Nature 23, 274–275
Cahen D (1983) Relative efficiency of flat plate solar collectors by transmission photoacoustics; importance of phase vibrations in signal generation. in ‘Photoacoustics, Principles and Applications’, Korpiun, P et al eds Viewey Verlag
Cahen D (1981) Photoacoustic figure of merit for photothermal energy conversion efficiency Opt Comm 39, 243–246
Buschmann C and Prehn H (1981) In vivo studies of radiative and non-radiative de-excitation processes of pigments in Raphanus by photoacoustic spectroscopy, Photochem Photobiophys 2, 209–215
Buschmann C and Prehn H (1983) In vivo photoacoustic spectra of Raphanus and Tradescantio leaves taken at different chopping frequencies of the excitation light. Photobiochem Photobiophys 5, 63–69
O'Hara EP, Tom R and Moore TA (1981) Absorption of light by pigments in lichens studied by photoacoustic spectroscopy in: Proc. 2nd Intern. Conf. on Photoacoustic Spectroscopy, Berkely, Calif. pp. B29/1 to B29/4
O'Hara EP, Tom RD and Moore TA (1983) Determination of the in vivo absorption and photosynthetic properties of the lichen Acarospora schleicheri using photoacoustic spectroscopy. Photochem Photobiol 38, 709–715
Galun M (1970) The Lichens of Israel. The Israel Academy of Science and Humanities. 120 pp. 28 plates, 2 maps
Galun M, Paran N and Ben-Shaul Y (1970) Ultrastructural studies of the fungus-alga association in Lecanora radiosa growing under different environmental conditions. J de Microsc 9, 801–806
Galun M (1979) Symbiotic relationship in plants. In: Dynamic aspects of host parasite relationships. Vol. III, Israel Univ Press Jerusalem 245 pp
Garty J and Fuchs C (1982) Heavy metals in the lichen Ramalina duriaei transplanted to biomonitoring stations. Water, Air and Soil Pollut 17, 175–183
Fuchs C and Garty J (1983) Elemental content in the lichen Ramalina duriaei (De Not.) Jatta at air quality biomonitoring stations. Env Exp Bot 23, 29–43
Ronen R and Galun M (1983) Pigments extraction from lichens with Dimethyl-Sulfoxide (DMSO) and estimation of chlorophyll degradation. Environmental and Experimental Botany, In press
Ronen R, Canaani O, Garty J, Cahen D, Malkin S and Galun M (1983) The effect of air-pollution and bisulfite treatment in the lichen Ramalina duriaei studied by photoacoustics. in Proc Sixth Int Congress of photosynthesis. In press
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Canaani, O., Ronen, R., Garty, J. et al. Photoacoustic study of the green alga Trebouxia in the lichen Ramalina duriaei in vivo . Photosynth Res 5, 297–306 (1984). https://doi.org/10.1007/BF00034975
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DOI: https://doi.org/10.1007/BF00034975