Abstract
Methionine sulfoximine provided at a concentration which inhibits photosynthesis in intact leaves (10 mM) had no significant influence on the rate of photosynthesis of isolated pea leaf chloroplasts. In contrast, ammonium, 3-(3,4-dichlorophenyl)-1,1-dimethylurea, and D,L-glyceraldehyde all strongly inhibited the photosynthesis of isolated chloroplasts. We conclude that low concentrations of methionine sulfoximine (up to 10 mM) have no direct effect on the photosynthetic process.
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References
Achhireddy N, Fletcher J and Beevers L (1982) Influence of methionine sulfoximine on the photosynthesis and ammonia accumulation by excised pepper leaves. Plant Physiol 65(Suppl.), 80
Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15
Crosthwaite LM and Sheen SJ (1979) Inhibition of ribuolse-1,5-bisphosphate carboxylase by a toxin isolated from Pseudomonas tabaci. Phytopathology 69: 376–379
Hall DO (1972) Nonmenclature for isolated chloroplasts. Nature New Biol 235 125–126
Keys AJ, Bird IF, Cornelius MJ, Lea PJ, Wallsgrove RM and Miflin BJ (1978) Photorespiratory nitrogen cycle. Nature 275:741–743
Miflin BJ and Lea PJ (1976) The pathway of nitrogen assimilation in plants. Phytochemistry 15:873–885
Mills WR and Joy KW (1980) A rapid method for isolation of purified, physiologically active chloroplasts, used to study the intracellular distribution of amino acids in pea leaves. Planta 148:75–83
Nakatani HY and Barber J (1977) An improved method for isolating chloroplasts retaining their outer membranes. Biochim. Biophys Acta 461:510–512
Nobel PS (1968) Chloroplast shrinkage and increased photophosphorylation in vitro upon illuminating intact plants of Pisum sativum. Biochim. Biophys. Acta 76: 638–641
Platt SG and Anthon GE (1981) Ammonia accumulation and inhibition of photosynthesis in methionine sulfoximine treated spinach. Plant Physiol. 67:509–513
Platt SG and Rand L (1982) Methionine sulfoximine effects on C4 plant leaf discs: Comparision with C3 species. Plant Cell Physiol. 23:917–922
Tate SS and Meister A (1973) Glutamine synthetases of mammalian liver and brain. In Prusiner S, Stadtman ER, eds. The enzymes of glutamine metabolism, pp. 77–127. New York: Academic Press
Walker DA (1980) Preparation of higher plant chloroplasts. In San Pietro A, ed. Methods in Enzymology, vol. 69, part C, pp 94–105, New York:Academic Press
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Muhitch, M.J., Fletcher, J.S. Influence of Methionine sulfoximine on the photosynthesis of isolated chloroplasts. Photosynth Res 4, 241–244 (1983). https://doi.org/10.1007/BF00052127
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DOI: https://doi.org/10.1007/BF00052127