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Hydrogen uptake by Azolla-Anabaena

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Summary

Experiments under laboratory conditions were carried out with the objective of studying hydrogen uptake in the Azolla-Anabaena system. Tritium was used as tracer and plants were incubated under different atmospheric composition: a) Air+3H2; b) Air+3H2+CO; c) Air+3H2+CO, d) Argon+3H2+CO2+CO and in presence and absence of light, to study hydrogen uptake via hydrogenase and nitrogenase activity.

Azolla-Anabaena showed greater hydrogen uptake under argon atmosphere than under air. Carbon monoxide decrease hydrogen uptake as well as nitrogenase activity. Under dark conditions, nitrogenase activity was smaller under argon than air atmosphere. Carbon monoxide decreased hydrogen uptake. H2-uptake was not affected by light, as it occurs under dark conditions. There are evidences of recycling of the hydrogen evolved through nitrogenase.

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Author notes

  1. A. P. Ruschel & J. R. de Freitas

    Present address: Centro Nacional de Pesquisa-Arroz, Feijão (CNPAF) EMBRAPA, Caixa Postal 179, 74000, Goiãnia, Goiás, Brazil

  2. P. M. da Silva (Graduate student)

    Present address: ESALQ, University of São Paulo, 13400, Piracicaba, SP, Brazil

Authors and Affiliations

  1. Centro de Energia Nuclear na Agricultura (CENA), University of São Paulo, Brazil

    A. P. Ruschel, J. R. de Freitas & P. M. da Silva (Graduate student)

Authors
  1. A. P. Ruschel
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  2. J. R. de Freitas
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  3. P. M. da Silva
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Additional information

This work was supported in part by International Atomic Energy Agency (IAEA) Austria and National Research Council (CNPq Brazil).

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Ruschel, A.P., de Freitas, J.R. & da Silva, P.M. Hydrogen uptake by Azolla-Anabaena. Plant Soil 97, 79–83 (1987). https://doi.org/10.1007/BF02149826

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  • DOI: https://doi.org/10.1007/BF02149826

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