Abstract
Nicotianamine is an intermediate for the biosynthesis of mugineic acid-family phytosiderophores (MAs) in the Gramineae and a key substance for iron metabolism in dicots. Nicotianamine synthase catalyzes the formation of nicotianamine from S-adenosylmethionine. Nicotianamine synthase activity was induced in barley roots at the 3rd day after withholding Fe supply and declined within one day followmg the supply of Fe3+-epihydroxymugineic acid. The induction of nicotianamine synthase activity by Fe-deficiency was observed also in sorghum, maize, and rye, and the level of nicotianamine synthase activity was highly associated with the MAs secreted among graminaceous plant tested. Therefore, the nicotianamine synthase gene may be a suitable candidate for making a transgenic plant tolerant to Fe-deficiency.
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Abbreviations
- p-APMSF:
-
(p-amidinophenyl) methanesulfonylfluoride hydrochloride
- NA:
-
nicotianamine
- DMA:
-
2′-deoxymugineic acid
- E-64:
-
trans-epoxysuccinyl-leucylamido-(4-guanidino) butane
- epiHMA:
-
3-epihydroxymugineic acid
- MAs:
-
mugineic acid-family phytosiderophores which include deoxymugineic acid, mugineic acid, hydroxymugineic acid, epihydroxymugineic acid and avenic acid
- PVP:
-
polyvinylpyrrolidone
- SAM:
-
S-adenosylmethionine
References
Fushiya S, Takahashi K, Nakatsuyama S, Sato Y, Nozoe S and Takagi S 1982 Co-occurrence of nicotianamine and avenic acids in Avena sativa and Oryza sativa. Phytochemistry 21, 1907–1908.
Higuchi K, Kanazawa K, Nishizawa N K, Chino M and Mori S 1994 Purification and characterization of nicotianamine synthase from Fe-deficient barley roots. Plant and Soil 165, 173–179.
Higuchi K, Nishizawa N K, Yamaguchi H, Römheld V, Marschner H and Mori S 1995 Response of nicotianamine synthase activity to Fe-deficiency is different between in tobacco plants and in barley plant. J. Exp. Bot. 46, 1061–1063.
Kanazawa K, Higuchi K, Nishizawa N K, Fushiya S, Chino M and Mori S 1994 Nicotianamine aminotransferase activities are correlated to the phytosiderophore secretions under Fe-deficient condition in Gramineae. J. Exp. Bot. 45, 1903–1906.
Lescure A M, Proudhon D, Pesey H, Regland M, Theil E C and Briat J F 1991 Ferritin gene transcription is regulated by iron in soybean cell cultures. Proc. Natl. Acad. Sci. USA 88, 8222–8226.
Ma J F and Nomoto K 1993 Two related biosynthetic pathways of mugineic acids in Gramineous plants. Plant Physiol. 102, 373–378.
Marschner H, Römheld V and Kissel M 1986 Different strategies in higher plants in mobilization and uptake of iron. J. Plant Nutr. 9, 695–713.
Mihashi S and Mori S 1989 Characterization of mugineic-acid-Fe transporter in Fe-deficient barley roots using the multicompartment transport box method. Biol. Metals 2, 146–154.
Mori S and Nishizawa N 1987 Methionine as a dominant precursor of phytosiderophore in Graminaceae plant. Plant Cell Physiol. 28, 1081–1092.
Mori S and Nishizawa N K 1989 Identification of barley chromosome no.4, possible encoder of genes of mugineic acid synthesis from 2′-deoxymugineic acid using wheat-barley addition lines. Plant Cell Physiol. 30, 1057–1061.
Mori S, Nishizawa N K and Fujigaki J 1990 Identification of rye chromosome 5R as a carrier of the genes for mugineic acid synthetase and 3-hydroxymugineic acid synthetase using wheat-rye addition lines. Jpn. J. Genet. 65, 343–352.
Mori S, Nishizawa N, Kawai S, Sato S and Takagi S 1987 Dynamic state of mugineic acid and analogous phytosiderophores in Fe-deficient barley. J. Plant Nutr. 10, 1003–1011.
Römheld V 1987 Different strategies for iron acquisition in higher plants. Physiol. Plant. 70, 231–234.
Scholz G, Becker R, Pich A and Stephan U W 1992 Nicotianaminea common constituent of strategies I and II of iron-acquisition by plants: A review. J. Plant Nutr. 15, 1647–1665.
Shojima S, Nishizawa N K and Mori S 1989a Establishment of a cell-free system for the biosynthesis of nicotianamine. Plant Cell Physiol. 30, 673–677.
Shojima S, Nishizawa N K, Fushiya S, Nozoe S, Kumashiro T, Nagata T, Ohata T and Mori S 1989b Biosynthesis of nicotianamine in the suspension-cultured cells of tobacco (Nicotiana megalosiphon). Biol. Metals 2, 142–145.
Shojima S, Nishizawa N K, Fushiya S, Nozoe S, Irifune T and Mori S 1990 Biosynthesis of phytosiderophores. Plant Physiol. 93, 1497–1503.
Singh K, Chino M, Nishizawa N K, Ohata T and Mori S 1993 Genotypic variation among Indian graminaceous species with respect to phytosiderophore secretion. In Genetic Aspects of Plant Mineral Nutrition Eds. P J Randall, E Delhaize, R A Richards and R Munns. pp 335–339. Kluwer Academic Publishers, Dordrecht, the Netherlands.
Takagi S 1976 Naturally occurring iron-chelating compounds in oat-and rice-root washing. I. Activity measurement and preliminary characterization. Soil Sci. Plant Nutr. 22, 4232–4233.
Takagi S, Nomoto K and Takemoto S 1984 Physiological aspect of mugineic acid, a possible phytosiderophore of graminaceous plants. J. Plant Nutr. 7, 469–477.
Walter A, Römheld V, Marschner H and Mori S 1994 Is the release of phytosiderophores in zinc-deficient wheat plants a response to impaired iron utilization? Physiol. Plant. 92, 493–500.
Yehuda Z, Shenker M, Römheld V, Hadar Y and Chen Y 1995 Efficient utilization of the siderophore rhizoferrin by plants. Abstract of the International Conference on BioIron (ICBI). April 16–21, 1995. pp 123. Asheville, North Carolina, USA.
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Higuchi, K., Kanazawa, K., Nishizawa, NK. et al. The role of nicotianamine synthase in response to Fe nutrition status in Gramineae. Plant Soil 178, 171–177 (1996). https://doi.org/10.1007/BF00011580
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DOI: https://doi.org/10.1007/BF00011580