Abstract
A number of strategies for identifying and cloning genes involved in nutrient transport and assimilation processes are discussed. Particular attention is paid to phenotypic screening by heterologous complementation of mutations which affect transport processes in other organisms and use of insertion elements in plant genomes.
A program in which complementation of yeast mutants is being used to clone genes involved in the uptake and assimilation of sulphate by plants is outlined. Information is provided on the isolation of yeast mutants and their characterization, choice of appropriate shuttle vectors, the construction of cDNA and genomic libraries, and screening of libraries for complementation.
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Smith, F.W., Hawkesford, M.J., Prosser, I.M. et al. Approaches to cloning genes encoding for nutrient transporters in plants. Plant Soil 155, 139–142 (1993). https://doi.org/10.1007/BF00025003
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DOI: https://doi.org/10.1007/BF00025003