Abstract
Nodulation tests onin-vitro propagated clones ofAlnus glutinosa ecotypes (forest ecotype, pioneer ecotype) withFrankia strains originating from both ecotypes indicated differences in host-plant compatibility. Inoculated plants of the pioneer ecotype clone were not infected by strains, that were unable to fix nitrogen in pure culture. Nodulation could only be induced on the clone of the forest ecotype, but no nitrogen-fixing activity could be detected. Ultra-structural observations of the nodules by SEM and TEM indicated that ineffectivity of these strains was correlated with the lack of vesicles in the infected cells. Cells were only filled with hyphae: neither sporangia nor vesicles could be detected. In contrast, effective nodules could be obtained on both alder clones after inoculation with an effective strain, showing normal development of vesicle clusters in infected cells. In pure culture the ineffective strains produced no vesicles; sporangia were found only during early stage of growth. The results demonstrate the existence ofFrankia strains which were either non-infective or ineffective on different clones ofAlnus glutinosa.
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References
Baker D, Newcombe W and Torrey J G 1980 Characterization of an ineffective actinorhizal microsymbiont,Frankia spp. Eull (Actinomycetales). Can. J. Microbiol. 26, 1072–1089.
Dawson O D and Gordon J C 1979 Nitrogen fixing in relation to photosynthesis inAlnus glutinosa. Bot. Gaz. 140(Suppl.), S70-S75.
Dawson J O and Sun S H 1981 The effect ofFrankia isolates fromComptonia peregrina andAlnus crispa on the growth ofAlnus glutinosa, A. cordata andA. incana clones. Can. J. For. Res. 11, 758–762.
Dijk C van and Sluimer-Stolk A 1984 An ineffective strain ofFronkia-AG in the dune area of Voorne. Progr. Rep. Inst. Ecol. Res. Verh. Kon. Ned. Akad. Wetensch., 2e Reeks 82, 56–59.
Dijk C van, Sluimer A and Weber A 1988 Host range differentiation of spore (+) and spore (−) strain types ofFrankia in stands ofAlnus glutinosa andAlnus incana in Finland. Physiol. Plant. (In press).
Dillon J T and Baker D 1982 Variation in nitrogenase activity among pure-culturedFrankia strains tested on actinorhizal plants as an indication of symbiotic compatibility. New Phytol. 92, 215–219.
Carton S, Hosier M A, Read P E and Farnham R S 1981 In vitro propagation ofAlnus glutinosa Gaertn. HortScience 16(6), 758–759.
Hall R B, McNabb H S jr, Maynard C A and Green T L 1979 Toward development of optimalAlnus glutinosa symbiosis. Bot Gaz 140(Suppl.), S120-S126.
Heller R 1953 Recherches sur la Nutrition Minérale des Tissues Végètaux Cultivésin vitro. Thése, Paris 223 pp.
Dechevalier M P, Baker D and Horrière F 1983 Physiology, chemistry, serology, and infectivity of twoFrankia isolates fromAlnus incana subsp.rugosa. Can. J. Bot. 61, 2826–2833.
Mackintosh A H and Bond G 1970 Diversity in the nodule endophytes of Alnus and Myrica. Phyton 27(1), 79–90.
Meesters T M 1987 Localization of nitrogenase in vesicles ofFrankia sp. Ccl.17 by immunogoldlabelling on ultrathin cryosections. Arch. Microbiol 146, 327–331.
Meesters T M, Genesen S Th van and Akkermans A D L 1985 Growth, acetylene reduction activity and localization of nitrogenase in relation to vesicle formation inFrankia strains Ccl.17 and Cpl.2. Arch. Microbiol. 143, 137–142.
Moss R D and Bard R C 1957 Physiological and biochemical technics.In The Society of American Bacteriologists (eds) Manual of Microbiology, Methods. McGraw-Hill, New York.
Murray M A, Zhang Z and Torrey J G 1985 Effect of O2 on vesicle formation, acetylene reduction, and O2 uptake kinetics inFrankia HFP CcI3 isolated fromCasuarina cunninghamiana. Can. J. Microbiol. 31, 804–809.
Noridge N A and Benson D R 1986 Isolation and nitrogen-fixing activity ofFrankia sp. CpI1 vesicles. J. Bacteriol. 166, 301–305.
Périnet P and Lalonde M 1983In vitro propagation and nodulation of the actinorhizal host plantAlnus glutinosa Gaertn. Plant Science Letters 29, 9–17.
Quispel A 1979 Growth and infectivity of the endophyte ofAlnus glutinosa.In Symbiotic Nitrogen Fixation in the Management of Temperate Forests. Eds. C J Gordon, C T Wheeler and D A Perry, pp 57–68. Oregon State University, Corvallis, Oregon.
Rodriguez-Barrueco C and Miguel C 1979 Host plant-endophyte specificity in actinomycete-nodulated plants.In Symbiotic Nitrogen Fixation in the Management of Temperate Forests. Eds. C J Gordon, C T Wheeler and D A Perry, pp 143–159. Oregon State University, Corvallis, Oregon.
Simon L, Stein A, Coté S and Lalonde M 1985 Performance of in vitro propagatedAlnus glutinosa (L.) Gaertn. clones inoculated withFrankiae. Plant and Soil 87, 125–133.
Tremblay F M and Lalonde M 1984 Requirements for in vitro propagation of seven nitrogen-fixingAlnus species. Plant Cell Tissue Organ Culture 3, 189–199.
VandenBosch K A and Torrey J G 1983 Host-endophyte interactions in effective and ineffective nodules induced by the endophyte ofMyrica gale. Can. J. Bot. 61, 2898–2909.
Verweij J A 1983 Een vergelÿkend onderzoek naar de kieming en vestiging van twee ecotypen van zwarte els (Alnus glutinosa (L.) Gaertn.). Rijksinstitut voor onderzoek in de bos- en landschapsbouw ‘De Dorschkamp’, Wageningen, nr. 354.
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Hahn, D., Starrenburg, M.J.C. & Akkermans, A.D.L. Variable compatibility of clonedAlnus glutinosa ecotypes against ineffectiveFrankia strains. Plant Soil 107, 233–243 (1988). https://doi.org/10.1007/BF02370552
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DOI: https://doi.org/10.1007/BF02370552