Abstract
We have recently characterized Nicotiana cytoplasmic (cyt) tRNAGCA Cys as novel UGA suppressor tRNA. Here we have isolated its corresponding (NtC1) and a variant (NtC2) gene from a genomic library of Nicotiana rustica. Both tRNACys genes are efficiently transcribed in HeLa cell nuclear extract and yield mature cyt tRNAsCys. Sequence analysis of the upstream region of the RAD51 single-copy gene of the Arabidopsis thaliana genome revealed a cluster of three tRNACys genes which have the same polarity and comprise highly similar flanking sequences. Of the three Arabidopsis tRNACys genes only one (i.e. AtC2) appears to code for a functional gene which exhibits an almost identical nucleotide sequence to NtC1. These are the first sequenced nuclear tDNAsCys of plant origin.
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References
ArendsS, KrausJ, BeierH: The tRNATyr multigene family of Triticum aestivum: genome organization, sequence analyses and maturation of intron-containing pre-tRNAs in wheat germ extract. FEBS Lett 384: 222–226 (1996).
BeierD, BeierH: Expression of variant nuclear Arabidopsis tRNASer genes and pre-tRNA maturating differ in HeLa, yeast and wheat germ extracts. Mol Gen Genet 233: 201–208 (1992).
BeierD, StangeN, GrossHJ, BeierH: Nuclear tRNATyr genes are highly amplified at a single chromosomal site in the genome of Arabidopsis thaliana. Mol Gen Genet 225: 72–80 (1991).
ClarksonSG: Transfer RNA genes. In: MacLeanN, Gregory SP, FlavellRA (eds) Eukaryotic Genes: Their Structure, Activity and Regulation, pp. 239–261. Butterworth, London (1983).
DignamJD, LebowitzRM, RoederRG: Accurate transcription initiation by polymerase II in a soluble extract from isolated mammalian nuclei. Nucl Acids Res 11: 1475–1489 (1983).
FuchsT, BeierD, BeierH: The tRNATyr multigene family of Nicotiana rustica: genome organization, sequence analyses and expression in vitro. Plant Mol Biol 20: 869–878 (1991).
GeiduschekEP, Tocchini-ValentiniGP: Transcription by RNA polymerase III. Annu Rev Biochem 57: 873–914 (1988).
KaçarY, BeierH, GrossHJ: The presence of tRNA pseudogenes in mammalia and plants and their absence in yeast may account for different specificities of pre-tRNA processing enzymes. Gene 156: 129–132 (1995).
StangeN, BeierH: A cell-free plant extract for accurate pe-tRNA processing, splicing and modification. EMBO J 6: 2811–2818 (1987).
StangeN, BeierD, BeierH: Expression of nuclear tRNATyr genes from Arabidopsis thaliana in HeLa cell and wheat germ extracts. Plant Mol Biol 16: 865–875 (1991).
TeichmannT, UrbanC, BeierH: The tRNASer-isoacceptors and their genes in Nicotiana rustica: genome organization, expression in vitro and sequence analyses. Plant Mol Biol 24: 889–901 (1994).
ThomannH-U, SchmutzlerC, HüdepohlU, BlowM, GrossHJ: Genes, variant genes and pseudogenes of the human tRNATyr gene family. Expression and pre-tRNA maturation in vitro. J Mol Biol 209: 505–523 (1989).
UrbanC, BeierH: Cysteine tRNAs of plant origin as novel UGA suppressors. Nucl Acids Res 23: 4591–4597 (1995).
VanTelgenHJ, vanLoonLC: Isolation and electrophoretic analysis of chromatin-associated proteins from virus-infected tobacco leaves. Z Pflanzenphysiol 112: 171–180 (1983).
VanTolH, StangeN, GrossHJ, BeierH: A human and a plant intron-containing tRNATyr gene are both transcribed in a HeLa cell extract but spliced along different pathways. EMBO J 6: 35–41 (1987).
WoodL, HatzenbuhlerN, PetersonR, VogeliG: Isolation of a mouse genomic clone containing four tRNACys-encoding genes. Gene 98: 249–252 (1991).
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Urban, C., Smith, K.N. & Beier, H. Nucleotide sequences of nuclear tRNACys genes from Nicotiana and Arabidopsis and expression in HeLa cell extract. Plant Mol Biol 32, 549–552 (1996). https://doi.org/10.1007/BF00019108
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DOI: https://doi.org/10.1007/BF00019108