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Nucleotide sequence of a cDNA clone encoding the precursor of the peridinin-chlorophyll a-binding protein from the dinoflagellate Symbiodinium sp.

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Abstract

mRNA from the dinoflagellate Symbiodinium sp. isolated from the staghorn coral Acropora formosa was used for the construction of cDNA libraries. A cDNA clone was identified which encoded the precursor of peridinin-chlorophyll a-binding protein (PCP), including a 52 amino acid transit peptide and the 313 amino acid mature protein. The deduced amino acid sequence clearly contains an internal duplication, implying that amongst dinoflagellates the M r 35 000 form of PCP has arisen by duplication and fusion of genes encoding the M r 15 000 form. This is the first reported sequence of a dinoflagellate light-harvesting protein. The anatomy of the mature protein and the transit peptide are discussed.

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Abbreviations

PCP:

peridinin-chlorophyll a-binding protein; cab, chlorophyll a/b-binding protein

LHC:

light-harvesting complex

FCP:

fucoxanthin-chlorophyll a/c-binding protein

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Authors and Affiliations

  1. Department of Molecular Sciences, James Cook University of North Queensland, 4811, Townsville, Queensland, Australia

    Belinda J. Norris & David J. Miller

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  1. Belinda J. Norris
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  2. David J. Miller
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Norris, B.J., Miller, D.J. Nucleotide sequence of a cDNA clone encoding the precursor of the peridinin-chlorophyll a-binding protein from the dinoflagellate Symbiodinium sp.. Plant Mol Biol 24, 673–677 (1994). https://doi.org/10.1007/BF00023563

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  • DOI: https://doi.org/10.1007/BF00023563

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