Abstract
In Arabidopsis tissues, the pool of tubulin protein is provided by the expression of multiple α-tubulin and β-tubulin genes. Previous evidence suggested that the TUA2 α-tubulin gene was expressed in all organs of mature plants. We now report a more detailed analysis of TUA2 expression during plant development. Chimeric genes containing TUA2 5′-flanking DNA fused to the β-glucuronidase (GUS) coding region were used to create transgenic Arabidopsis plants. Second-generation progeny of regenerated plants were analyzed by histochemical assay to localize GUS expression. GUS activity was seen throughout plant development and in nearly all tissues. The blue product of GUS activity accumulated to the highest levels in tissues with actively dividing and elongating cells. GUS activity was not detected in a few plant tissues, suggesting that, though widely expressed, the TUA2 promoter is not constitutively active.
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References
Carpenter JL, Ploense SE, Snustad DP, Silflow CD: Preferential expression of an α-tubulin gene of Arabidopsis in pollen. Plant Cell 4: 557–571 (1992).
Derksen J, Emons AM: Microtubules in tip growth systems. In: Heath IB (ed) Tip Growth in Plant and Fungal Cells, pp. 147–181. Academic Press, New York (1990).
Falconer MM, Seagull RW: Immunofluorescent and calcofluor white staining of developing tracheary elements in Zinnia elegans L. suspension cultures. Protoplasma 125: 190–198 (1985).
Falconer MM, Seagull RW: Xylogenesis in tissue culture II. Microtubules, cell shape and secondary wall patterns. Protoplasma 133: 140–148 (1986).
Gunning BES, Hardham AR: Microtubules. Annu Rev Plant Physiol 33: 651–698 (1982).
Hepler PK, Palevitz BA: Microtubules and microfilaments. Annu Rev Plant Physiol 25: 309–362 (1974).
Jefferson RA, Kavanagh TA, Bevan MW: GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901–3907 (1987).
Kopczak SD, Haas NA, Hussey PJ, Silflow CD, Snustad DP: The small genome of Arabidopsis contains at least six expressed α-tubulin genes. Plant Cell 4: 539–547 (1992).
Lazo GR, Stein PA, Ludwig RR: A transformation competent Arabidopsis genomic library in Agrobacterium. Bio/Technology 9: 963–967 (1991).
Lloyd CW: The plant cytoskeleton: The impact of fluorescence microscopy. Annu Rev Plant Physiol 38: 119–139 (1987).
Ludwig SR, Oppenheimer DG, Silflow CD, Snustad DP: Characterization of the α-tubulin gene family of Arabidopsis thaliana. Proc Natl Acad Sci USA 84: 5833–5837 (1987).
Ludwig SR, Oppenheimer DG, Silflow CD, Snustad DP: The α1-tubulin gene of Arabidopsis thaliana: Primary structure and preferential expression in flowers. Plant Mol Biol 10: 311–321 (1988).
Oppenheimer DG, Haas N, Silflow CD, Snustad DP: The β-tubulin gene family of Arabidopsis thaliana: Preferential accumulation of the β1 transcript in roots. Gene 63: 87–102 (1988).
Pierson ES, Cresti M: Cytoskeleton and cytoplasmic organization of pollen and pollen tubes. Int Rev Cytol 140: 73–125 (1992).
Snustad DP, Haas NA, Kopczak SD, Silflow CD: The small genome of Arabidopsis contains at least nine expressed β-tubulin genes. Plant Cell 4: 549–556 (1992).
Valvekens D, VanMontagu M, vanLijsebettens M: Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection. Proc Natl Acad Sci USA 85: 5536–5540 (1988).
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Carpenter, J.L., Kopczak, S.D., Snustad, D.P. et al. Semi-constitutive expression of an Arabidopsis thaliana α-tubulin gene. Plant Mol Biol 21, 937–942 (1993). https://doi.org/10.1007/BF00027126
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DOI: https://doi.org/10.1007/BF00027126