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Isolation and sequence of a tomato cDNA clone encoding subunit II of the photosystem I reaction center

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Abstract

We report here the isolation and nucleotide sequence of a cDNA clone encoding a phtosystem I polypeptide that is recognized by a polyclonal antibody prepared against subunit II of the photosystem I reaction center. The transit peptide processing site was determined to occur after Met50 by N terminal sequencing. The decuced sequence of this protein predicts that the polypeptide has a net positive charge (pI=9.6) and no membrane spanning regions are evident from the hydropathy plot. Based on these considerations and the fact that subunit II is solubilized by alkali treatment of thylakoids, we concluded that subunit II is an extrinsic membrane protein. The absence of hydrophobic regions characteristic of thylakoid transfer domains furthermore implies that subunit II is localized on the stromal side of the membrane.

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Author notes

  1. Neil E. Hoffman

    Present address: Department of Plant Biology, Carnegie Institute of Washington, 290 Panama Street, 94305, Stanford, CA, USA

Authors and Affiliations

  1. Plant Science Institute, Department of Biology, University of Pennsylvania, 19104, Philadelphia, PA, USA

    Neil E. Hoffman, Kenton Ko & Anthony R. Cashmore

  2. Department of Biology, University of Michigan, 48109, Ann Arbor, MI, USA

    Eran Pichersky

  3. Philip Morris Research Center, P.O. Box 26583, 23261, Richmond, VA, USA

    Vedpal S. Malik

Authors
  1. Neil E. Hoffman
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  2. Eran Pichersky
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  3. Vedpal S. Malik
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  4. Kenton Ko
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  5. Anthony R. Cashmore
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Hoffman, N.E., Pichersky, E., Malik, V.S. et al. Isolation and sequence of a tomato cDNA clone encoding subunit II of the photosystem I reaction center. Plant Mol Biol 10, 435–445 (1988). https://doi.org/10.1007/BF00014949

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  • DOI: https://doi.org/10.1007/BF00014949

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