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Differentiation of the double membrane system during ascospore-maturation of Arthroderma vanbreuseghemii as revealed by periodic acid-alkaline bismuth staining

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Abstract

Asci of Arthroderma vanbreuseghemii were investigated by electron microscopy using periodic acid-alkaline bismuth (PABi) staining. At the early stage of ascosporogenesis, PABi-reactive substance was present homogeneously on both the inner and outer tracks of the double membrane system. Immediately after ascospore-delimitation, this substance gradually decreased and ultimately disappeared from the outer track. In contrast, the inner membrane was persistently loaded with the PABi-reactive material. These observations suggest that at this stage the outer membrane of the double membrane system begins to differentiate into the ascospore cell wall, utilizing its carbohydrate constituent.

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References

  1. Ainsworth, S. K., S. Ito, & M. J. Karnovsky, 1972. Alkaline bismuth reagent for high resolution ultrastructural demonstration of periodate-reactive sites. J. Histochem. Cytochem. 20: 995–1005.

    Google Scholar 

  2. Alexopoulos, C. J. & C. W. Mims, 1979. Subdivision ascomycotina class ascomycetes. In Introductory Mycology, pp. 235–258. Wiley, New York.

    Google Scholar 

  3. Beckett, A., 1981. Ascospore formation. In: Turian, G. (ed.) The Fungal Spore, pp. 107–129. Academic Press, New York.

    Google Scholar 

  4. Black, S. H. & C. Gorman, 1971. The cytology of Hansenula. III. Nuclear segregation and envelopement during ascosporogenesis in Hansenula wingei. Arch. Microbiol. 79: 231–248.

    Google Scholar 

  5. Hill, T. W., 1975. Ultrastructure of ascosporogenesis in Nannizzia gypsea. J. Bacteriol. 112: 743–748.

    Google Scholar 

  6. Spurr, A. R., 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruc. Res. 26: 31–43.

    Google Scholar 

  7. Takashio, M., 1973. Etudedes phénomènes de reproduction liés au viellissement et au rajeunissement des cultures de champignons. Ann. Soc. Bel. Méd. Tropic. 53: 427–580.

    Google Scholar 

  8. Tanaka, S., T. Fujigaki & S. Watanabe, 1982. Ultrastructure of the concentric membrane system in asci of Arthroderma vanbreuseghemii. Sabouraudia 20: 127–136.

    Google Scholar 

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Authors and Affiliations

  1. Department of Dermatology, Shiga University of Medical Science, Seta Tsukinowa-cho, 520-21, Otsu, Japan

    S. Tanaka, T. Fujigaki & S. Watanabe

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  1. S. Tanaka
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  2. T. Fujigaki
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  3. S. Watanabe
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Tanaka, S., Fujigaki, T. & Watanabe, S. Differentiation of the double membrane system during ascospore-maturation of Arthroderma vanbreuseghemii as revealed by periodic acid-alkaline bismuth staining. Mycopathologia 86, 55–58 (1984). https://doi.org/10.1007/BF00437230

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