Abstract
We have isolated and sequenced one intergenic region and a small part of the flanking regions (18S and 26S rRNA coding regions) of the rRNA-encoding genes (rDNA) from the sea urchinParacentrotus lividus. This region is about 3.8 Kb long. Northern blot hybridizations and S1 mapping experiments demonstrated the presence of a partially processed 21S rRNA precursor which has the same 5′ terminus as the 32S primary precursor, also in developmental stages characterized by a low rate of rRNA synthesis.
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Abbreviations
- bp:
-
base pair(s)
- Kb:
-
Kilobase(s) or 1000 bp
- nt:
-
nucleotide(s)
- rDNA:
-
DNA encoding rRNA
- rRNA:
-
ribosomal RNA
- S:
-
sedimentation constant
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Cantone, M., Barbieri, R., Duro, G. et al. Sequence analysis of the rDNA spacer ofParacentrotus lividus and observations about pre-rRNA processing. Mol Biol Rep 18, 177–182 (1993). https://doi.org/10.1007/BF01674428
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DOI: https://doi.org/10.1007/BF01674428