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Initiation of histone synthesis by f-met-tRNAf in an ascites cell-free system

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Abstract

Formyl-[35S]methionine is incorporated into histones synthesized by a mouse ascites cell-free system supplemented with histone mRNA and f-[35S]met-tRNAf from yeast. Most of the [35S]methionine incorporated can be shown to be at the N-terminus by Edman degradation after deformylation. This indicates that methionine can be used as the initiator amino acid for histones in this cell-free system.

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Authors and Affiliations

  1. Department of Biology, Massachusetts Institute of Technology, 02139, Cambridge, Mass., USA

    M. Jacobs-Lorena & C. Baglioni

  2. Laboratory of Cell Biology, Rome, Italy

    M. Jacobs-Lorena & C. Baglioni

Authors
  1. M. Jacobs-Lorena
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  2. C. Baglioni
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Jacobs-Lorena, M., Baglioni, C. Initiation of histone synthesis by f-met-tRNAf in an ascites cell-free system. Molecular Biology Reports 1, 113–117 (1973). https://doi.org/10.1007/BF00357590

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  • DOI: https://doi.org/10.1007/BF00357590

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