Skip to main content
SpringerLink
Log in

Purification of thrombospondin from human platelets

  • Preparation Of Cell Adhesive Receptors
  • Published:
Journal of tissue culture methods

Summary

Thrombospondins are a rapidly growing family of adhesive proteins that have diverse activities to modulate cellular growth, motility, and gene expression. Thrombospondin-1 (TSP) was the first identified member of this family and is the major form of thrombospondin in human platelets. A method is described to prepare TSP from human platelets in biologically active form with minimal degradation or contamination with other platelet proteins.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Adams J, Lawler J (1993). The thrombospondin family. Curr Biol 3: 188–190.

    PubMed  Google Scholar 

  2. Anstall HS, Urie PM (1986). A manual of hemotherapy. New York: John Wiley and Sons, pp 82–89.

    Google Scholar 

  3. Bornstein P (1992). Thrombospondins: Structure and regulation of expression. FASEB J 6: 3290–3299.

    PubMed  Google Scholar 

  4. Clezardin P, McGregor JL, Manach M, Robert F, Dechvanne M, Clemetson KJ (1984). Isolation of thrombospondin released from thrombin stimulated human platelets by fast protein liquid chromatography on an ion exchange Mono Q column. J Chromatogr 296: 249–256.

    PubMed  Google Scholar 

  5. Florence TM (1980). Degradation of protein disulfide bonds in dilute alkali. Biochem J 189: 507–520.

    PubMed  Google Scholar 

  6. Lawler J, Simons ER (1983). Cooperative binding of calcium to thrombospondin. J Biol Chem 258: 12098–12101.

    PubMed  Google Scholar 

  7. Lawler JW, Slayter HS, Coligan JE (1978). Isolation and characterization of a high molecular weight glycoprotein from human blood platelets. J Biol Chem 253: 8609–8616.

    PubMed  Google Scholar 

  8. Murphy-Ullrich JE, Schultz-Cherry S, Höök M (1992). Transforming growth factor-β complexes with thrombospondin. Mol Biol Cell 3: 181–188.

    PubMed  Google Scholar 

  9. Roberts DD, Haverstick DM, Dixit VM, Frazier WA, Santoro SA, Ginsburg V (1985). The platelet glycoprotein thrombospondin binds specifically to sulfatides. J Biol Chem 260: 9405–9411.

    PubMed  Google Scholar 

  10. Santoro SA, Frazier WA (1987). Isolation and characterization of thrombospondin. Meth Enzymol 144: 438–446.

    PubMed  Google Scholar 

  11. Sun X, Mosher DF (1990). Contamination of thrombospondin with vitronectin. Blood 76: 1666–1668.

    PubMed  Google Scholar 

  12. Sun X, Skorstengaard K, Mosher DF (1992). Disulfides modulate RGD-inhibitable cell adhesive activity of thrombospondin. J Cell Biol 118: 693–701.

    PubMed  Google Scholar 

  13. Speziale MV, Detwiler TC (1990). Free thiols of platelet thrombospondin: Evidence for disulfide isomerization. J Biol Chem 265: 17859–17867.

    PubMed  Google Scholar 

  14. Tuszynski GP, Srivastava S, Switalska HI, Holt JC, Cierniewski CS, Niewiarowski S (1985). The interaction of human platelet thrombospondin with fibrinogen: Thrombospondin purification and specificity of interaction. J Biol Chem 260: 12240–12245.

    PubMed  Google Scholar 

  15. Vogel T, Blake DA, Whikehart DR, Guo N-H, Zabrenetzky VS, Roberts DD (1993). Specific simple sugars promote chemotaxis and chemokinesis of corneal endothelial cells. J Cell Physiol 157: 359–366.

    PubMed  Google Scholar 

  16. Vuillard L, Clezardin P, Miller A (1991). Models of human platelet thrombospondin in solution: A dynamic light scattering study. Biochem J 275: 263–266.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA

    David D. Roberts, JoAnne Cashel & Neng-hua Guo

Authors
  1. David D. Roberts
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. JoAnne Cashel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Neng-hua Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to David D. Roberts.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Roberts, D.D., Cashel, J. & Guo, Nh. Purification of thrombospondin from human platelets. Journal of Tissue Culture Methods 16, 217–222 (1994). https://doi.org/10.1007/BF01540654

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01540654

Key words

Search

Navigation