Summary
The use of chemoselective ligation methods and orthogonal protection techniques allows access to Template-Assembled Synthetic Protein (TASP) molecules exhibiting a large variety of packing topologies. This is demonstrated for the synthesis of an antiparallel 4α-helical bundle TASP by condensing amphiphilic peptide blocks, containing aldehyde functions at the C- or N-terminus, to a selectively addressable topological template via oxime bond formation. The resulting antiparallel 4α-helix TASP is obtained in high yield and shows a template-induced helical conformation.
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Nyanguile, O., Mutter, M. & Tuchscherer, G. Synthesis of antiparallel 4α-helix bundle TASP by chemoselective ligation. Lett Pept Sci 1, 9–16 (1994). https://doi.org/10.1007/BF00132757
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DOI: https://doi.org/10.1007/BF00132757