Abstract
The HPLC separation of fluorescent o-phtaldialdehyde (OPA) derivatives has been applied to the assay of free amino acids from five microalgae commonly used in aquaculture: Tetraselmis suecica, Skeletonema costatum, Chaetoceros calcitrans, Thalassiosira sp. and Isochrysis galbana, as part an assessment of their potential use in cosmetic products. Thirteen free amino acids were analyzed using High Performance Liquid Chromatography. There were considerable differences between species. However, four amino acids were responsible for more than 60% total concentration in all species: ASP, GLU, ARG and TYR; the next most important (accounting for less than 30%) were: ALA, VAL, PHE and LYS.
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References
Chretiennot-Dinet MJ, Robert R, His E (1986) Utilisation des 'algues fourrage' en aquaculture. Ann. Biol. 25: 97–119.
Coiffard L, Alliot A, Piron M (1992) Seasonal and geographical adaptation of Crithmum maritimum L.: variations in inorganic content. Int. J. Cosmetic. Sci. 15: 261–269.
Coiffard L, De Roeck-Holtzhauer Y (1995) Variations géographiques et saisonnières de la teneur en protéines et en acides aminés de Crithmum maritimum L. (Apiacées). Acta Bot. Gallica 142: 405–407.
Cooper JDM, Turnell DC (1982) Fluorescence detection of cystine by o.phthaldialdehyde derivation and its separation using high performance liquid chromatography. J. Chromatogr. 227: 158–161.
Corre O (1983) Acides aminés libres Résultats observés pour l'épiderme et le film surfacique. Thesis of Doctorate in Pharmacy, University of Nantes, France.
De Roeck-Holtzhauer Y, Quere I, Claire C (1991) Vitamin analysis of five planktonic microalgae and one macroalga. J. appl. Phycol. 3: 259–264.
Fürst P, Pollack L, Graser TA, Godel H, Stehle P (1990) Appraisal of four pre-column derivatization methods for the high-performance liquid chromatographic determination of the free amino acids in biological materials. J. Chromatogr. 499: 557–569.
Herbreteau F, Coiffard L, Derrien A, De Roeck-Holtzhauer Y (1994) Détermination de la composition des acides gras de la chair de crépidule (Crepidula fornicata L., Calyptraeidae). Rev. Fr. Corps gras 5/6: 119–121.
Lindroth P, Mopper K (1979) High performance liquid chromatography determination of subpicomole amounts of amino acids by precolumn fluorescence derivatization with o.phthaldialdehyde. Analyt. Chem. 51: 1667–1674.
Martin F (1982) Détermination d'acides aminés libres dans les tissus végétaux par chromatographie liquide à haute performance (HPLC) des dérivés o.phthaldialdéhyde. Cah. Chromatog. 5: 20–33.
Martin-Jézéquel V, Poulet SA, Harris RP, Moal J, Samain JF (1988) Interspecific and intraspecific composition and variation of free amino acids in marine phytoplankton. Mar. Ecol. Progr. Ser. 44: 303–313.
Peschet MJ, Jacob F, Perrier J (1985) Dosage des acides aminés des proteines de levure par chromatographie liquide à haute performance: Application de la méthode des dérivés orthphtaldialdehyde. Cah. Chromatogr. 10: 16–26.
Servel MO, Claire C, Derrien A, Coiffard L, De Roeck-Holtzhauer Y (1994) Fatty acid composition of some marine microalgea. Phytochemistry 36: 691–693.
Weerner JA, Dejong JC, Lenstra JB (1951) Effects of free amino acids on the skin. Dermatologica 103: 1–9.
Wickfors GH, Twarog JW, Ukeles R (1984) Influence of chemical composition of algal food sources on growth of juvenile oysters, Crassostrea virginica. Biol. Bull. 167: 251–263.
Wiszniak J (1978) Introduction à l'étude de la cosmétologie I Les paramètres à mesurer. Cutis 2: 839–853.
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Derrien, A., Coiffard, L.J.M., Coiffard, C. et al. Free amino acid analysis of five microalgae. Journal of Applied Phycology 10, 131–134 (1998). https://doi.org/10.1023/A:1008003016458
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DOI: https://doi.org/10.1023/A:1008003016458