Abstract
We adapted a probiotic culture system originally developed for domestic animals and fish for the intensive culture of rotifers. In this modification, a crude starter fluid was made by incubating a mixture of some 40 species of bacteria (so-called Uchishiro Microorganisms) with the by-products of other food-related materials. The probiotic culture medium (PCM) was then completed by incubation of 50 g of starter fluid (25 °C) for 3–4 days in 1 L of 50% sea water with strong aeration. After processing, the medium may be used as food for the rotifer Brachionus plicatilis. We determined the optimal levels for feeding B. plicatilis cultures by providing a starter culture of 100 rotifers different concentrations of PCM and monitoring their population growth over five days. The highest population density of B. plicatilis (351 ind ml-1) was achieved from a mixture of 20 ml PCM per 1 L rotifer water. From these experiments, we conclude that PCM is useful as food for rotifer culture if it is supplied at a satisfactory concentration.
Similar content being viewed by others
References
Hirata, H., 1996. Probiotic culture of fish. Yoshoku 33(3): 142–145.
Hirata, H. & Y. Mori, 1967. Culture of rotifer Brachionus plicatilis fed on baker's yeast. Saibaigyogyo 5(2): 36–40.
Hirata, H., M. Ushiro & I. Hirata, 1982. Ecological succession of Chlorella saccarophila, Brachionus plicatilis, and autogenous bacteria in culture water. Mem. Fac. Fish., Kagoshima Univ. 31: 153–160.
Kosasa, M., 1990. Recent works on the probiotics for feeding in domestic animals. Feeding 30(7): 41–47.
Nakano, M., 1996. Studies on the probiotic culture of flounder. Proc. Symp. Ecol. Aquacul. 1–4.
Ushiro, M. & A. Hino, 1990. Microbial consumption by the rotifer Brachionus plicatilis. Kaiyo 22(1): 20–27.
Yamasaki, S. & H. Hirata, 1990. Relationship between food consumption and metabolism of rotifer Brachionus plicatilis. Nippon Suisan Gakkaishi 56: 591–594.
Yamasaki, S. & H. Hirata, 1993. Water quality maintenance and food recycling the waste matter of a rotifer ecocystem culture. Suisanzoshoku 41: 7–11.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hirata, H., Murata, O., Yamada, S. et al. Probiotic culture of the rotifer Brachionus plicatilis. Hydrobiologia 387, 495–498 (1998). https://doi.org/10.1023/A:1017004124600
Issue Date:
DOI: https://doi.org/10.1023/A:1017004124600