Abstract
In order to determine the suitability of thecyprinodont fish Aphanius dispar as a biologicalcontrol agent for mosquito larvae, the effects oftemperature, salinity, feeding rate and photoperiod onits reproductive condition were studied. The factorsexamined were the gonado-somatic index (GSI) and fourdistinct stages of cell maturation in the ovaries(oogonia, primary oocytes, secondary oocytes andmature ova). The fish were found to be tolerant to awide range of the examined factors. GSI's were notaffected by ambient temperatures over the range of18 °C to 37 °C. However, oocyte maturationstages increased as temperatures rose from 18 °Cto 27 °C, with mature ova first being found at thelatter temperature. No significant differences amongmaturation stages were found between27 °C–37 °C. At salinities ranging from of0 ppt to 56 ppt, no significant differences were found betweenmean GSI's, except for the two extremes of the testrange. The ovaries of fish kept in distilled watercontained primordial germ cells only. In feedingexperiments, significant differences were found onlybetween the ovaries (GSI's and oocyte maturationstages) of fish deprived of food and those fed at≥ 1% body weight per day. Regarding photoperiod,a decrease in day length from 14L:10D (the longest dayof the year) to 10L:14D (the shortest) caused adecrease in oocyte maturation stages. Fish at theintermediate level (12L:12D) still had ovariessuitable for spawning, whereas those at 10L:14D didnot. The results of this study show that limitinglevels for adequate reproductive condition were foundfor some of the factors investigated, however, rangesof the factors above these levels were relativelylarge. That Aphanius dispar's reproductivecondition may be controlled in an artificialenvironment is essential for it to be mass produced,and a prerequisite for its use in the biologicalcontrol of mosquito larvae.
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References
Ali, M. A. (ed.). 1980. Environmental Physiology of Fishes. Plenum Press. New York, 941 pp.
Bein, R. & G. Ribi. 1994. Effects of larval density and salinity on the development of perch larvae Perca fluviatilis(L). Aquat. Sci. 56: 97–105.
Byniak, E., 1979. The effect of day length and temperature on the reproductive cycle of the female Aphanius mento(Heckel), (Cyprinodontiformes, Teleostei). (Doctoral Thesis) Zoology Dept., Hebrew University, Jerusalem, 69 pp. (In Hebrew).
Castleberry, D. T. & J. J. Cech Jr., 1990. Mosquito control in wastewater: A controlled and quantitative comparison of pup-fish (Cyprinodon nevadensis amargose), mosquitofish (Gambusia affinis) and guppies (Poecilia reticulata) in Sago pondweed marshes. J. am. Mosquito Contr. Ass. 6: 223–228.
Degani, G., 1989. The effect of temperature, light, fish size and container size on breeding of Trichogaster trichopterus. Isr. J. Aquacult. Bamidgeh. 41: 67–73.
Egami, N., 1957. Record of the number of eggs obtained from a single pair of Oryzias latipeskept in laboratory aquaria. Journal of the Faculty of Science. Tokyo Univ. Sec. IV: 521–538.
Fishelson, L., 1975. Ethology and reproduction of pteroid fishes found in the Gulf of Aqaba (Red Sea), especially Dendrochirus brachypterus(Cuvier) (Pteroidae, Teleosti). Pubblizioni Stazioni Zoologie de Napoli. 39 Suppl. 635–656.
Fletcher, M., A. Teklehaimanot & G. Yemane. 1992. Control ofmosquito larvae in the port city of Assab by an indigenous larvivorous fish, Aphanius dispar. Acta Trop. 52: 155–166.
Fletcher, M., A. Teklehaimanot, G. Yemane, A. Kassahun, G. Kidane & Y. Beyene. 1993. Prospects for the use of larvivorous fish for malaria control in Ethiopia; search for indigenous species and evaluation of their feeding capacity for mosquito larvae. J. trop. Med. Hygiene 96: 12–21.
Francis, M. P., 1994. Duration of larval and spawning periods in Pagrus auratus(Sparidae) determined from otolith daily increments. Envir. Biol. Fish. 39: 137–152.
Frenkel, V., 1995. The effects of environmental factors on the growth and reproduction of the fish Aphanius dispar(Rüppell, 1828). (Masters Thesis) Zool. Dept., Tel Aviv, University, Tel Aviv: 88 pp.
Goren, M., 1983. The freshwater fishes of Israel. Biology and Taxonomy. Hakibutz Hamehuchad Publishing House. Tel Aviv, 102 pp (in Hebrew).
Goren, M., D. Homski & A. Gasith, 1988. The use of some fish species for biological control of mosquito larvae under various ecological conditions. p. 103. The Sixth Congress of European Ichthyological Union. Budapest, Hungary. (Abstract).
Guiguemde, T. R., F. Dao, V. Curtis, A. Traore, B. Sondo, J. Testa & J. B. Ouedrago. 1994. Household expenditure on malaria prevention and treatment for families in the town of Bobo-Dioulasso, Burkina Faso. Trans. r. Soc. trop. Diseases & Hygiene 88: 285–287.
Guillory, V. & W. E. Johnson, 1986. Habitat conservation status and zoogeography of the cyprinodont fish Cyprinodon variegatus hubbsi(Carr). Southw. Nat. 31: 95–100.
Haas, R., 1982. Notes on the ecology of Aphanius dispar(Pisces, Cyprinodontidae) in the Sultanate of Oman. Freshwat. Biol. 12: 89–95.
Haas, R. & R. Pal, 1984. Mosquito Larvivorous Fishes. Bull. ent. Soc. Am. 30: 1–25.
Hepher, B., 1988. Nutrition of Pond Fishes. Cambridge University Press. Cambridge, 388 pp.
Hoar, W. S., D. J. Randall & E. M. Donaldson (eds), 1983a. Fish Physiology. Vol. I. Excretion, Ionic Regulation and Metabolism, 465 pp.
Hoar, W. S., D. J. Randall & E. M. Donaldson (eds), 1983b. Fish Physiology. Vol. IX. Reproduction. Part A. Endocrine Tissues and Hormones, 483 pp.
Homski, D., M. Goren & A. Gasith, 1994. Comparative evaluation of the larvivorous fish Gambusia affinisand Aphanius disparas mosquito control agents. Hydrobiologia 284: 137–146.
Jones, J. C., 1978. The feeding behavior of mosquitoes. Sci. Am. 238: 112–120.
Kock, K. H., S. Wilhelms, I. Everson & J. Groger, 1994. Variations in the diet composition and feeding intensity of mackerel icefish Champsocephalus gunnariat South Georgia (Antarctic). Mar. Ecol. Prog. Ser. 108: 43–57.
Lagler, K. F., J. E. Bardach & R. R. Miller. 1962. Ichthyology. John Wiley and Sons, Inc., New York, 545 pp.
Lichtenberg, E. R. & W. Getz, 1985. Economics of rice-field mosquito control in California. Bioscience 35: 292–297.
Lotan, R., 1973. Osmoregulation during adaptation to fresh water in the euryhaline teleost Aphanius disparRüppell (Cyprinodontidae, Pisces). J. comp. Physiol. 87: 339–349.
Lotan, R., 1982. The killifish Aphanius dispar. Israel, Land and Nature 8.
Marshall, N. B., 1970. The life of fishes. Universe Books. New York, 402 pp.
Martin, F. D., 1972. Factors influencing the local distribution of Cyprinodon variegatus(Pisces: Cyprinodontidae). Trans. am. Fish. Soc. 1972: 87–93.
Mendlen, A. B., 1951. Preliminary observations on the effects of light and temperature upon the reproduction in Gambusia affinis. Copeia 2: 148–152.
Nasir, A. S., 1979. History of the use of larvivorous fish for larval control in Somalia. W.H.O. EM/TR. SMR. LRV. FSH. MSQ. CTR/ Somalia, 3 pp.
Nordlie, F. G., S. J. Walsh, D. C. Haney & T. F. Nordlie, 1991. The influence of ambient salinity on routine metabolism in the teleost Cyprinodon variegatusLacepede. J. Fish Biol. 38: 115–122.
Pant, C. P., N. Rishikesh, Y. H. Bang & A. Smith, 1981. Progress in malaria vector control. W.H.O Bull. 59: 325–333.
Paperna, I., 1984. Reproduction cycle and tolerance to temperature and salinity of Amyloodinium ocellatum(Brown,1931) (Dinoflagellida). Ann. Parasitol. Humaine & Comparee 59: 7–30.
Persson, P., K. Sundell & B. T. Bjornsson, 1994. Estradiol-17 beta (E2) induced calcium uptake and reabsorption in juvenile rainbow trout Oncorhynchus mykiss. Fish Physiol. Biochem. 13: 379–386.
Purdom, C. E., 1993. Genetics and Fish Breeding. Chapman & Hall. London, Glasgow, 277 pp.
Ritchie, S. A. & C. Laidlawbell, 1994. Do fish repel oviposition of Aedes taeniorhynchus. J. am. Mosquito Contr. Assoc. 10: 3.
Roberts, R. J., 1989. Fish Pathology. Bailliere Tindall. London, 466 pp.
Schmidt-Nielson, K., 1975. Animal Physiology. Adaption and Environment. Cambridge University Press, 699 pp.
Shepherd, C. J. & N. R. Bromage, 1988. Intensive Fish Farming. BSP Professional Books. Oxford, 404 pp.
Walters, L. L. & E. F. Lenger, 1979. Impact of the desert pupfish Cyprinodon maculariusand Gambusia affinis affinison fauna in pond ecosystems. Hilgardia. 48: 1–18.
Wang, N. & R. Eckmann, 1994. Effects of temperature and food density on egg development, larval survival and growth of perch Perca fluviatilis(L.). Aquaculture. 122: 323–333.
Whitehead, P. J. P., M. L. Bauchot, J. C. Hureau, J. Nielsen & E. Tortnese (eds), 1986. Fishes of the North-eastern Atlantic and the Mediterranean. Vol. II. UNESCO. Paris, 490 pp.
Zaman, M. S., 1980. Malaria control through fish. Pakistan J. Sci. 32: 163–168.
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Frenkel, V., Goren, M. Some environmental factors affecting the reproduction of Aphanius dispar (Rüppell, 1828). Hydrobiologia 347, 197–207 (1997). https://doi.org/10.1023/A:1003000225293
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DOI: https://doi.org/10.1023/A:1003000225293