Skip to main content
SpringerLink
Log in

An electro-chromatographic investigation of the amino acid pattern of some marine algae from Alexandria

  • Published:
Hydrobiologia Aims and scope Submit manuscript

Summary

  1. 1.

    An electro-chromatographic method was developed for the separation and identification of 26 different amino acids. This method proved to be time saving and most suitable for routine analyses in physicochemical work.

  2. 2.

    This technique was applied in studying the amino acid pattern of algal extracts of nine common species representing Chlorophyta, Phaeophyta and Rhodophyta, from Alexandria shore.

  3. 3.

    A semi-quantitative method, based on visual comparison of intensity of colour and size of spots with standardized records, was described and used in this investigation.

  4. 4.

    The comparative results showed that the highest amino acid content was found in Pterocladia, followed by Ulva and Dictyota for the protein fraction; Codium, Pterocladia and Enteromorpha for the peptide fraction, and Pterocladia, Hypnea and Codium for free amino acids.

  5. 5.

    Each alga has its own amino acid pattern in the proteins, peptides and free state; but qualitative differences were less significant than quantitative variations, which may not conform to the general pattern of the group.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Biserte, G., Dautrevaux, M. & Boulanger, P. - 1963 - La chromato-électrophorèse des acides amines et des peptides. Bull. Soc. chim. de France 3917: 2954–2959

    Google Scholar 

  • Coulson, C. B. - 1953 - Proteins of marine algae. Chem. Ind. 1953: 997–998.

    Google Scholar 

  • Dekker, C. A., Stone, D. & Fruton, J. S. - 1949 - A peptide from a marine alga. J. biol. Chem. 181: 719–729.

    CAS  PubMed  Google Scholar 

  • Fowden, L. - 1962- In “Physiology and Biochemistry of Algae.” Academic Press, New York & London, 189 p.

    Google Scholar 

  • Hass, P. - 1950 - On certain peptides occurring in marine algae. Biochem. J. 46: 503–505.

    Article  Google Scholar 

  • Hass, P., Hill, T. G. & Russell-Wells, B. - 1938 - On certain simple peptides occurring in marine algae. Biochem. J. 32: 2129–2133.

    Article  Google Scholar 

  • Kuon, J. & Bernhard, R. A.,-1963 - An examination of the free amino acids of the common onion. J. Food Sci. 28: 298–304.

    Article  CAS  Google Scholar 

  • Makisumi, S. - 1959 - Occurrence of ariginylglutamine in a green alga, Cladophora sp. J. Biochem. (Tokyo) 46: 63–71.

    CAS  Google Scholar 

  • Smith, I. - 1953 - Colour reactions on paper chromatograms by a dipping technique. Nature (London) 171: 43–44.

    Article  CAS  Google Scholar 

  • Tagaki, M. & Suzuki, M. - 1952 - Chemical studies on marine algae. IV. The amino acids containing sulphur. Bull. Fac. Fish., Hokkaido Univ. 2: 293–296.

    Google Scholar 

  • Zweig, G. - 1956 - Preservation of ninhydrin-amino acid chromatograms. Anal. Chem. 28: 428.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Botany Department, Faculty of Science, Alexandria University, Egypt

    A. H. Nasr, I. A. Bekheet & R. K. Ibrahim

Authors
  1. A. H. Nasr
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. A. Bekheet
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. K. Ibrahim
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nasr, A.H., Bekheet, I.A. & Ibrahim, R.K. An electro-chromatographic investigation of the amino acid pattern of some marine algae from Alexandria. Hydrobiologia 29, 80–92 (1967). https://doi.org/10.1007/BF00142055

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00142055

Keywords

Search

Navigation