Abstract
Ethylene and polyamine metabolism, both sharing a common precursor, S-adenosylmethionine (SAM), were investigated during detached tomato (Lycopersicon esculentum Mill. nothovar F1 “Lorena”) fruit ripening. Putrescine (PUT) was found to be the major polyamine in the fruits, always over 100 nmols/g FW, while spermidine (SPD) was between 7% and 3% of the level of PUT. Spermine (SPM) was not detected at any stage of ripening. The level of PUT and SPD, did not change significantly during ripening in spite of the almost continuous synthesis of 1-aminocyclopropane-1-carboxylic acid (ACC), the ethylene precursor, and only at the last stage of ripening was a drastic decrease in SPD content observed. The results obtained show that the onset of ACC synthesis and its accumulation within the tissue is not a consequence of a decrease in SPD synthesis.
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Apelbaum A, Burgoon AC, Anderson JD, Lieberman M, Ben-Arie R and Mattoo AK (1981) Polyamines inhibit biosynthesis of ethylene in higher plant tissue and fruit protoplasts. Plant Physiol 68: 453–456
Atta-Aly MA, Saltveit MEJr and Hobson GE (1987) Effect of silver ions on ethylene biosynthesis by tomato fruit tissue. Plant Physiol 83: 44–48
Bakanashvili M, Barkai-Golan R, Kopeliovitch E and Apelbaum A (1987) Polyamine biosynthesis in Rhizopus-infected tomato fruits: Possible interaction with ethylene. Physiol Mol Plant Pathol 31: 41–50
Brecht JK (1987) Locular gel formation in developing tomato fruit and the initiation of ethylene production. HortScience 22: 476–479
Bufler G (1986) Ethylene-promoted conversion of 1-aminocyclopropane-1-carboxylic acid to ethylene in peel of apple at various stages of fruit development. Plant Physiol 80: 539–543
Dibble ARG, Davies PJ and Mutschler MA (1988) Polyamine content of long-keeping alcobaca tomato fruit. Plant Physiol 86: 338–340
Even-Chen Z, Mattoo AK and Goren R (1982) Inhibition of ethylene biosynthesis by aminoethoxyvinylglycine and by polyamines shunts label from 3,4-[14C]Methionine into spermidine in aged orange peel discs. Plant Physiol 69: 385–388
Flores HE and Galston AW (1982) Analysis of polyamines in higher plants by high performance liquid chromatography. Plant Physiol 69: 701–706
Galston AW and Kaur-Sawhney R (1987) Polyamines and senescence in plants. In: WW Thompson, EA Nothnagel and R.C. Huffaker, eds. Plant Senescence: Its Biochemistry and Physiology, 167–181. The American Society of Plant Physiologists
Grierson D (1985) The gene expression in ripening tomato fruit. CRC Critical Reviews in Plant Sciences 3: 113–132
Hoffman NE and Yang SF (1980) Changes of 1-aminocyclopropane-1-carboxylic acid content in ripening fruits in relation to their ethylene evolution rates. J Am Soc Hortic Sci 105: 492–495
Hoffman NE, Liu Y and Yang SF (1983) Changes in 1-(malonylamino)cyclopropane-1-carboxylic acid content in wilted wheat leaves in relation to their ethylene production rates and 1-aminocyclopropane-1-carboxylic acid content. Planta 157: 518–523
Hyodo H and Tanaka K (1986) Inhibition of 1-aminocyclopropane-1-carboxylic acid synthase activity by polyamines, their related compounds and metabolites of S-adenosylmethionine. Plant and Cell Physiol 27: 389–391
Icekson I, Goldslust A and Apelbaum A (1985) Influence of ethylene on S-adenosylmethionine decarboxylase activity in etiolated pea seedlings. J Plant Physiol 119: 335–345
Jeffery D, Smith C, Goodenough P, Prosser I and Grierson D (1984) Ethylene-independent and ethylene-dependent biochemical changes in ripening tomatoes. Plant Physiol 74: 32–38
Kagan-Zur V and Mizrahi Y (1987) Fruit ripening in tetraploid tomato (Lycopersicon esculentum Mill.). J Hortic Sci 62: 243–248
Ke D and Romani RJ (1988) Effects of spermidine on ethylene production and the senescence of suspension-cultured pear fruit cells. Plant Physiol Biochem 26: 109–116
Kende H and Boller T (1981) Wound ethylene and 1-aminocyclopropane-1-carboxylate synthase in ripening tomato fruit. Planta 151: 476–481
Kushad MM, Yelenosky G and Knight R (1988) Interrelationship of polyamine and ethylene biosynthesis during avocado fruit development and ripening. Plant Physiol 87: 463–467
Lizada MCC and Yang SF (1979) A simple and sensitive assay for 1-aminocyclopropane-1-carboxylic acid. Anal Biochem 100: 140–145
Mansour R, Latché A, Vaillant V, Pech JC and Reid MS (1986) Metabolism of 1-aminocyclopropane-1-carboxylic acid in ripening apple fruits. Physiol Plant 66: 495–502
Miyazaki JH and Yang SF (1987) The methionine salvage pathway in relation to ethylene and polyamine biosynthesis. Physiol Plant 69: 366–370
Redmond JW and Tseng A (1979) High pressure liquid chromatographic determination of putrescine, cadaverine, spermidine and spermine. J Chromatogr 170: 479–481
Smith TA (1985) Polyamines. Ann Rev Plant Physiol 36: 117–143
Su LY, McKeon T, Grierson D, Cantwell M and Yang SF (1984) Development of 1-aminocyclopropane-1-carboxylic acid synthase and polygalacturonase activities during the maturation and ripening of tomato fruit. HortScience 19: 576–578
Suttle JC (1981) Effect of polyamines on ethylene production. Phytochemistry 20: 1477–1480
Toumadje A and Richardson D (1988) Endogenous polyamine concentrations during development, storage and ripening of pear fruits. Phytochemistry 27: 335–338
Winer L and Apelbaum A (1986) Involvement of polyamines in the development and ripening of avocado fruits. J Plant Physiol 126: 223–233
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Casas, J.L., Acosta, M., Del Rio, J.A. et al. Ethylene evolution during ripening of detached tomato fruit: Its relation with polyamine metabolism. Plant Growth Regul 9, 89–96 (1990). https://doi.org/10.1007/BF00027436
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DOI: https://doi.org/10.1007/BF00027436