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Indole-3-butyric acid in plant growth and development

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Abstract

Within the last ten years it has been established by GC-MS thatindole-3-butyric acid (IBA) is an endogenous compound in a variety ofplant species. When applied exogenously, IBA has a variety of differenteffects on plant growth and development, but the compound is stillmainly used for the induction of adventitious roots. Using moleculartechniques, several genes have been isolated that are induced duringadventitious root formation by IBA. The biosynthesis of IBA in maize(Zea mays L.) involves IAA as the direct precursor. Microsomalmembranes from maize are able to convert IAA to IBA using ATP andacetyl-CoA as cofactors. The enzyme catalyzing this reaction wascharacterized from maize seedlings and partially purified. The invitro biosynthesis of IBA seems to be regulated by several externaland internal factors: i) Microsomal membranes from light-grownmaize seedlings directly synthesize IBA, whereas microsomal membranesfrom dark-grown maize plants release an as yet unknown reaction product,which is converted to IBA in a second step. ii) Drought and osmoticstress increase the biosynthesis of IBA maybe via the increaseof endogenous ABA, because application of ABA also results in elevatedlevels of IBA. iii) IBA synthesis is specifically increased byherbicides of the sethoxydim group. iv) IBA and IBA synthesizingactivity are enhanced during the colonization of maize roots with themycorrhizal fungus Glomus intraradices. The role of IBA forcertain developmental processes in plants is discussed and somearguments presented that IBA is per se an auxin and does notact via the conversion to IAA.

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References

  1. Alvarez R, Nissen SJ and Sutter EG (1989) Relationship between indole-3-acetic acid levels in apple (Malus pumila Mill) rootstocks cultured in vitro and adventitious root formation in the presence of indole-3-butyric acid. Plant Physiol 89: 439-443

    Google Scholar 

  2. Babczinski P and Fischer R (1991) Inhibition of acetylcoenzyme A carboxylase by the novel grass-selective herbicide 3-(2,4-dichlorophenyl)-perhydroindolizine-2,4-dione. Pestic Sci 33: 455-466

    Google Scholar 

  3. Badenoch-Jones J, Summons RE, Rolfe BG and Letham DS (1984) Phytohormones, Rhizobium mutants and nodulation in legumes. IV. Auxin metabolism in pea root nodules. J Plant Growth Regul 3: 23-29

    Google Scholar 

  4. Baraldi R, Cohen JD, Bertazza D and Predieri S (1993) Uptake and metabolism of indole-3-butyric acid during the in vitro rooting phase in pear cultivars (Pyrus communis L.). Acta Hort 329: 289-291

    Google Scholar 

  5. Bartel B (1997) Auxin biosynthesis. Annu Rev Plant Physiol Plant Mol Biol 48: 51-66

    Google Scholar 

  6. Biddington NL and Dearman AS (1982) The effect of abscisic acid on root and shoot growth of cauliflower plants. Plant Growth Regul 1: 15-24

    Google Scholar 

  7. Blazkova A, Sotta B, Tranvan H, Maldiney R, Bonnet M, Einhorn JH, Kerhoas L and Miginiac E (1997) Auxin metabolism and rooting in young and mature clones of Sequoia sempervirens. Physiol Plant 99: 73-80

    Google Scholar 

  8. Blommaert KLJ (1954) Growth-and inhibiting substances in relation to the rest period of the potato tuber. Nature 174: 970-972

    Google Scholar 

  9. Buchala AJ and Schmid A (1979) Vitamin D and its analogues as a new class of plant growth substances affecting rhizogenesis. Nature 280: 230-231

    Google Scholar 

  10. Burton JD, Gronwald JW, Somers DA, Gengenbach BG and Wyse DL (1989) Inhibition of corn acetyl-CoA carboxylase by cyclohexanedione and aryloxyphenoxypropionate herbicides. Pestic Biochem Physiol 34: 76-85

    Google Scholar 

  11. Caboche M, Muller J-F, Chanut F, Aranda G and Cirakoglu S (1987) Comparison of the growth promoting activities and toxicities of various auxin analogs on cells derived from wildtype and a non-rooting mutant of tobacco. Plant Physiol 83: 795-800

    Google Scholar 

  12. Cabrera HL, Poch C, Peto CA and Chory J (1993) A mutation in the Arabidopsis DET3 gene uncouples photoregulated leaf development from gene expression and chloroplast biogenesis. Plant J 4: 671-682

    Google Scholar 

  13. Chory J and Peto CA (1990) Mutations in the DET1 gene affect cell-type-specific expression of light-regulated genes and chloroplast development in Arabidopsis. Proc Natl Acad Sci USA 87: 8776-8780

    Google Scholar 

  14. Chory J, Nagpal P and Peto CA (1991) Phenotypic and genetic analysis of det2, a new mutant that affects light-regulated seedling development in Arabidopsis. Plant Cell 3: 445-460

    Google Scholar 

  15. Cohen JD and Bandurski RS (1982) Chemistry and physiology of the bound auxins. Annu Rev Plant Physiol 33: 403-430

    Google Scholar 

  16. Cohen JD, Baldi BG and Slovin JP (1986) 13C6-[Benzene ring]-indole-3-acetic acid. Plant Physiol 80: 14-19

    Google Scholar 

  17. Cooper WC (1935) Hormones in relation to root formation on stem cuttings. Plant Physiol 10: 789-794

    Google Scholar 

  18. CosgroveDJandLiZC1993Roleofexpansinincellenlargementofoatcoleoptiles.Analysisofdevelopmentalgradientsandphotocontrol.PlantPhysiol 103: 1321-132

    Google Scholar 

  19. Dannenberg G, Latus C, Zimmer W, Hundeshagen B, Schneider-Poetsch H-J and Bothe H (1992) Influence of vesicular-arbuscular mycorrhiza on phytohormone balances in maize (Zea mays L.). J Plant Physiol 141: 33-39

    Google Scholar 

  20. Davies PJ (ed.) (1995) Plant Hormones. Physiology, Biochemistry and Molecular Biology. Dordrecht, The Netherlands: Kluwer Academic Publishers. ISBN 0-7923-2985-6

    Google Scholar 

  21. Dunberg A, Hsihan S and Sandberg G (1981) Auxin dynamics and the rooting of cuttings of Pinus sylvestris. Plant Physiol Suppl 67: 5

    Google Scholar 

  22. Epstein E and Lavee S (1984) Conversion of indole-3-butyric acid to indole-3-acetic acid by cuttings of grapevine (Vitis vinifera) and olive (Olea europea). Plant Cell Physiol 25: 697-703

    Google Scholar 

  23. Epstein E, Muszkat L and Cohen JD (1988) Identification of indole-3-butyric acid (IBA) in leaves of cypress and maize by gas-chromatography-mass spectrometry. Alon HaNotea 42: 917-919

    Google Scholar 

  24. Epstein E, Chen K-H and Cohen JD (1989) Identification of indole-3-butyric acid as an endogenous constituent of maize kernels and leaves. Plant Growth Regul 8: 215-223

    Google Scholar 

  25. Epstein E, Nissen SJ and Sutter EG (1991) Indole-3-acetic acid and indole-3-butyric acid in tissues of carrot inoculated with Agrobacterium rhizogenes. J. Plant Growth Regul. 10: 97-100

    Google Scholar 

  26. Epstein E and Sagee O (1992) Effect of ethylene treatment on transport and metabolism of indole-3-butyric acid in citrus leaf midribs. Plant Growth Regul 11: 357-362

    Google Scholar 

  27. Epstein E and Ackerman A (1993) Transport and metabolism of indole-3-butyric acid in cuttings of Leucadendron discolor. Plant Growth Regul 12: 17-22

    Google Scholar 

  28. Epstein E and Ludwig-Müller J (1993) Indole-3-butyric acid in plants: occurrence, biosynthesis, metabolism, and transport. Physiol Plant 88: 382-389

    Google Scholar 

  29. Epstein E, Sagee O and Zelcer A (1993) Uptake and metabolism of indole-3-butyric acid and indole-3-acetic acid by Petunia cell suspension cultures. Plant Growth Regul 13: 31-40

    Google Scholar 

  30. Epstein E, Zilkah S, Faingersh G and Rotebaum A (1993) Transport and metabolism of indole-3-butyric acid in sterile easy-to-root and difficult-to-root cuttings of sweet cherry (Prunus avium L.). Acta Hort 329: 292-295

    Google Scholar 

  31. Ernstsen A and Sandberg G (1986) Identification of 4-chloroindole-3-acetic acid and indole-3-aldehyde in seeds of Pinus sylvestris. Physiol Plant 68: 511-518

    Google Scholar 

  32. Esch H, Hundeshagen B, Schneider-Poetsch H-J and Bothe H (1994) Demonstration of abscisic acid in spores and hyphae of the arbuscular-mycorrhizal fungus Glomus and in the N2-fixing cyanobacterium Anabaena variabilis. Plant Sci 99: 9-16

    Google Scholar 

  33. Fallik E, Okon Y, Epstein E, Goldman A and Fischer M(1989) Identification and quantification of IAA and IBA in Azospirillum brasilense-inoculated maize roots. Soil Biol Biochem 21: 147-153

    Google Scholar 

  34. Harbage JF and Stimart DP (1996) Effect of pH and 1H-indole-3-butyric acid (IBA) on rooting of apple microcuttings. J Amer Soc Hort Sci 121: 1049-1053

    Google Scholar 

  35. Hartmann HT, Kester DE and Davies FT (1990) Plant Propagation: Principles and Practices. Englewood Cliffs, NJ: Prentice-Hall, pp 246-247

    Google Scholar 

  36. Hartung W and Heilmeier H (1992) The development of the root system of the winter annual desert plant Anastatica hierochuntica L. The significance of soil water content and nutrient supply. Flora 186: 117-125

    Google Scholar 

  37. Hartung W, Zhang J and Davies WJ (1994) Does abscisic acid play a stress physiological role in maize plants growing in heavily compacted soil? J Exp Bot 45: 221-226

    Google Scholar 

  38. Hobbie LJ (1998) Auxin: Molecular genetic approaches in Arabidopsis. Plant Physiol Biochem 36: 91-102

    Google Scholar 

  39. Hertel R, Evans ML, Leopold AC and Sell HM (1969) The specificity of the auxin transport system. Planta 85: 238-249

    Google Scholar 

  40. Hutchison KW, Singer PB, McInnis S, Diaz-Sala C and Greenwood MS (1999) Expansins are conserved in conifers and expressed in hypocotyls in response to exogenous auxin. Plant Physiol 120: 827-832

    Google Scholar 

  41. Kaldorf M and Ludwig-Müller J (2000) AM fungi might affect the root morphology of maize by increasing indole-3-butyric acid biosynthesis. Physiol Plant 109: 58-67

    Google Scholar 

  42. Katayama M, Kato K, Kimoto H and Fujii S (1995) (S)-(+)-4,4,4-trifluoro-3-(indole-3-) butyric acid, a novel fluorinated plant growth regulator. Experientia 51: 721-724

    Google Scholar 

  43. Katayama M and Gautam RK (1996) Synthesis and biological activities of substituted 4,4,4-trifluoro-3-(indole-3-)butyric acids, novel fluorinated plant growth regulators. Biosci Biotech Biochem 60: 755-759

    Google Scholar 

  44. Koornneef M, Jorna ML, Brinkhorst-van der Swan DLC and Karssen CM (1982) The isolation of abscisic acid (ABA) deficient mutant by selection of induced revertants in nongerminating gibberellin sensitive lines of Arabidopsis thaliana (L.) Heynh. Theor Appl Genet 61: 385-393

    Google Scholar 

  45. Kreps JA and Town CD (1992) Isolation and characterization of a mutant of Arabidopsis thaliana resistant to alpha methyl tryptophan. Plant Physiol 99: 269-275

    Google Scholar 

  46. Kreps JA, Ponappa T, Dong W and Town CD (1996) Molecular basis of α-methyltryptophan resistance in amt-1, a mutant of Arabidopsis thaliana with altered tryptophan metabolism. Plant Physiol 110: 1159-1165

    Google Scholar 

  47. Lange T (1997) Cloning gibberellin dioxygenase genes from pumpkin endosperm by heterologous expression of enzyme activities in Escherichia coli. Proc Natl Acad Sci USA 94: 6553-6558

    Google Scholar 

  48. LaTorre KA, Harris DM and Rundle SJ (1997) Differential expression of three Arabidopsis genes encoding the B' regulatory subunit of protein phosphatase 2A. Eur J Biochem 245: 156-163

    Google Scholar 

  49. Lemieux B, Miquel M, Somerville C and Browse J (1990) Mutants of Arabidopsis with alterations in seed lipid fatty acid composition. Theor Appl Genet 80: 234-240

    Google Scholar 

  50. Leyser O (1997) Auxin: Lessons from a mutant weed. Physiol Plant 100: 407-414

    Google Scholar 

  51. Ludwig-Müller J (1999) The biosynthesis of auxins. Curr. Topics Plant Biol. 1: 77-88

    Google Scholar 

  52. Ludwig-Müller J and Epstein E (1991) Occurrence and in vivo biosynthesis of indole-3-butyric acid in corn (Zea mays L.). Plant Physiol 97: 765-770

    Google Scholar 

  53. Ludwig-Müller J and Epstein E (1992) Indole-3-acetic acid is converted to indole-3-butyric acid by seedlings of Zea mays L. In: Karssen CM, van Loon LC and Vreugdenhil D (eds) Progress in Plant Growth Regulation. Boston, MA: Kluwer Academic Publishers, pp 188-193. ISBN 0-7923-1617-7

    Google Scholar 

  54. Ludwig-Müller J and Epstein E (1993) Indole-3-butyric acid in Arabidopsis thaliana. II. In vivo metabolism. Plant Growth Regulation 13: 189-195

    Google Scholar 

  55. Ludwig-Müller J, Sass S, Sutter EG, Wodner M and Epstein E (1993) Indole-3-butyric acid in Arabidopsis thaliana. I. Identification and quantification. Plant Growth Regul 13: 179-187

    Google Scholar 

  56. Ludwig-Müller J and Epstein E (1994) Indole-3-butyric acid in Arabidopsis thaliana. III. In vivo biosynthesis. Plant Growth Regul 14: 7-14

    Google Scholar 

  57. Ludwig-Müller J and Hilgenberg W (1995) Characterization and partial purification of indole-3-butyric acid synthetase from maize (Zea mays). Physiol Plant 94: 651-660

    Google Scholar 

  58. Ludwig-Müller J, Hilgenberg W and Epstein E (1995) The in vitro biosynthesis of indole-3-butyric acid in maize. Phytochemistry 40: 61-68

    Google Scholar 

  59. Ludwig-Müller J, Raisig A and Hilgenberg W (1995) Uptake and transport of indole-3-butyric acid in Arabidopsis thaliana: Comparison with other natural and synthetic auxins. J Plant Physiol 147: 351-354

    Google Scholar 

  60. Ludwig-Müller J, Schubert B and Pieper K (1995) Regulation of IBA synthetase by drought stress and abscisic acid. J Exp Bot 46: 423-432

    Google Scholar 

  61. Ludwig-Müller J, Kaldorf M, Sutter EG and Epstein E (1997) Indole-3-butyric acid (IBA) is enhanced in young maize (Zea mays L.) roots colonized with the arbuscular mycorrhizal fungus Glomus intraradices. Plant Sci 125: 153-162

    Google Scholar 

  62. Ludwig-Müller J, Schubert B, Rademacher W and Hilgenberg W (2000) Indole-3-butyric acid biosynthesis in maize is enhanced by cyclohexanedione herbicides. Physiol Plant, in press

  63. Lund ST, Smith AG and Hackett WP (1996) Cuttings of a tobacco mutant, rac, undergo cell division but do not initiate adventitious roots in response to exogenous auxin. Physiol Plant 97: 372-380

    Google Scholar 

  64. McCready CC (1963) Movement of growth regulators in plants. I. Polar transport of 2,4-D in segments from petioles of Phaseolus vulgaris. New. Phytol. 62: 3-18

    Google Scholar 

  65. McGaw BA (1995) Cytokinin biosynthesis and metabolism. In: Davies PJ (ed.) Plant Hormones. Physiology, Biochemistry and Molecular Biology. The Netherlands: Kluwer Academic Publishers, pp 98-117. ISBN 0-7923-2985-6

    Google Scholar 

  66. McQueen-Mason S (1995) Expansins and cell wall expansion. J Exp Bot 46: 1639-1650

    Google Scholar 

  67. Merckelbach C, Buchala AJ and Meier H (1991) Adventitious rooting in cuttings of Populus tremula: Metabolism of IAA and IBA. Abstr. 14th Int. Conf. on Plant Growth Substances (Amsterdam, The Netherlands), p 21

  68. Mirza JI, Olsen GM, Iversen TH and Maher EP (1984) The growth and gravitropic responses of wild-type and auxinresistant mutants of Arabidopsis thaliana. Physiol Plant 60: 516-522

    Google Scholar 

  69. Neuteboom LW, Ng JM, Kuyper M, Clijdesdale OR, Hooykaas PJ and van der Zaal BJ (1999) Isolation and characterization of cDNA clones corresponding with mRNAs that accumulate during auxin-induced lateral root formation. Plant Mol Biol 39: 273-287

    Google Scholar 

  70. Neuteboom LW, Veth-Tello LM, Clijdesdale OR, Hooykaas PJ and van der Zaal BJ (1999) A novel subtilisin-like protease gene from Arabidopsis thaliana is expressed at sites of lateral root emergence. DNA Res 6: 13-19

    Google Scholar 

  71. Nordström A-C, Jacobs FA and Eliasson L (1991) Effect of exogenous indole-3-acetic acid and indole-3-butyric acid on internal levels of the respective auxins and their conjugation with aspartic acid during adventitious root formation in pea cuttings. Plant Physiol 96: 856-861

    Google Scholar 

  72. Normanly J, Slovin JP and Cohen JD (1995) Rethinking auxin biosynthesis and metabolism. Plant Physiol 107: 323-329

    Google Scholar 

  73. Östin A, Ilic' N and Cohen JD (1999) An in vitro system from maize seedlings for tryptophan-independent indole-3-acetic acid biosynthesis. Plant Physiol 119: 173-178

    Google Scholar 

  74. Ozga JA and Reinecke DM (1998) Interaction of 4-chloroindole-3-acetic acid and gibberellins in early pea fruit development. Plant Growth Regul 27: 33-38

    Google Scholar 

  75. Pan R and Gui H (1997) Physiolgical basis of the synergistic effects of IBA and triadimefon on rooting of mung bean hypocotyls. Plant Growth Regul 22: 7-11

    Google Scholar 

  76. Poupart J and Waddell C (1999) Characterization of rib1, an IBA Specific Mutant of Arabidopsis. Symposia on Plant Hormones, University of Missouri, April 14-17

  77. Pythoud F and Buchala AJ (1989) The fate of vitamin D3 and indolebutyric acid applied to cuttings of Populus tremula L. during adventitious root formation. Plant Cell Environ 2: 489-494

    Google Scholar 

  78. Reinecke DM (1999) 4-Chloroindole-3-acetic acid and plant growth. Plant Growth Regul 27: 3-13

    Google Scholar 

  79. Reinecke DM, Ozga JA and Magnus V (1995) Effect of halogen substitution of indole-3-acetic acid on biological activity in pea fruit. Phytochemistry 40: 1361-1366

    Google Scholar 

  80. Richmond TA and Bleecker AB (1999) A defect in β-oxidation causes abnormal inflorescence development in Arabidopsis. Plant Cell 11: 1911-1923

    Google Scholar 

  81. Riov J and Goren R (1979) Effect of ethylene on auxin transport and metabolism in midrib sections in relation to leaf abscission of woody plants. Plant Cell Environ 2: 83-89

    Google Scholar 

  82. Riov J and Yang SF (1989) Ethylene and auxin-ethylene interaction in adventitious root formation in mung bean (Vigna radiata) cuttings. J Plant Growth Regul 8: 131-141

    Google Scholar 

  83. Robertson JM, Hubick KT, Yeung EC and Reid DM (1990) Developmental responses to drought and abscisic acid in sun-flower roots. I. Root growth, apical anatomy and osmotic adjustment. J Exp Bot 41: 325-337

    Google Scholar 

  84. Sakagami Y, Manabe K, Aitani T, Thiruvikraman SV and Marumo S (1993) L-4-chlorotryptophan from immature seeds of Pisum sativum and reassignment of the absolute stereo230 chemistry of N-malonyl-4-chlorotryptophan. Tetrahedron Lett 43: 1057-1060

    Google Scholar 

  85. Schneider EA, Kazakoff CW and Wightman F (1985) Gas chromatography-mass spectrometry evidence for several endogenous auxins in pea seedling organs. Planta 165: 232-241

    Google Scholar 

  86. Shoseyov L, Sutter EG, Epstein E and Shoseyov O (1989) IBA induces β-1,3-glucanase activity in 20-day-old mung bean cuttings. Plant Growth Soc Am Quarterly 17: 92

    Google Scholar 

  87. Smith RD and Walker JC (1996) Plant protein phosphatases. Annu Rev Plant Physiol Plant Mol Biol 47: 101-125

    Google Scholar 

  88. Sponsel VM (1995) Gibberellin biosynthesis and metabolism. In: Davies PJ (ed.) Plant Hormones. Physiology, Biochemistry and Molecular Biology. The Netherlands: Kluwer Academic Publishers, pp 66-97. ISBN 0-7923-2985-6

    Google Scholar 

  89. Sutter EG and Cohen JD (1992) Measurement of indolebutyric acid in plant tissues by isotope dilution gas chromatographymass spectrometry analysis. Plant Physiol 99: 1719-1722

    Google Scholar 

  90. Tisserant B, Gianinazzi S and Gianinazzi-Pearson V (1996) Relationships between lateral root order, arbuscular mycorrhiza development and the physiological state of the symbiotic fungus in Platanus acerifolia. Can J Bot 74: 1947-1955

    Google Scholar 

  91. van der Krieken WM, Breteler H and Visser MHM (1992) The effect of conversion of indolebutyric acid into indoleacetic acid on root formation. Plant Cell Physiol 33: 709-713

    Google Scholar 

  92. van der Krieken WM, Breteler H, Visser MHM and Mavridou D (1993) The role of the conversion of IBA into IAA on root generation in apple: introduction of a test system. Plant Cell Rep 12: 203-206

    Google Scholar 

  93. Wakil SJ (1989) Fatty acid synthase, a proficient multifunctional enzyme. Biochemistry 28: 4523-4530

    Google Scholar 

  94. Wightman F and Lighty DL (1982) Identification of phenylacetic acid as a natural auxin in the shoots of higher plants. Physiol Plant 55: 17-24

    Google Scholar 

  95. Wiesman Z, Riov J and Epstein E (1988) Comparison of movement and metabolism of indole-3-acetic acid in mung bean cuttings. Physiol Plant 74: 556-560

    Google Scholar 

  96. Wiesman Z, Riov J and Epstein E (1989) Characterization and rooting ability of indole-3-butyric acid conjugates formed during rooting of mung bean cuttings. Plant Physiol 91: 1080-1084

    Google Scholar 

  97. Wright AD, Simpson MB, Neuffer MG, Michalczuk L, Slovin JP and Cohen JD (1991) Indole-3-acetic acid biosynthesis in the mutant orange pericarp, a tryptophan auxotroph. Science 254: 998-1000

    Google Scholar 

  98. Yano K, Yamauchi A and Kono Y (1996) Localized alteration in lateral root development in roots colonized by an arbuscular mycorrhizal fungus. Mycorrhiza 6: 409-415

    Google Scholar 

  99. Zimmerman PW and Wilcoxon F (1935) Several chemical growth substances which cause initiation of roots and other responses in plants. Contrib. Boyce Thompson Inst 7: 209-229

    Google Scholar 

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  1. Botanisches Institut, Fakultät Mathematik und Naturwissenschaften, Technische Universität Dresden, Zellescher Weg 22, 01062, Dresden, Germany

    Jutta Ludwig-Müller

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Ludwig-Müller, J. Indole-3-butyric acid in plant growth and development. Plant Growth Regulation 32, 219–230 (2000). https://doi.org/10.1023/A:1010746806891

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