Abstract
Vegetative propagation through rooting in stem cuttings in five tree mangroves namely Bruguiera parviflora, Cynometra iripa, Excoecaria agallocha, Heritiera fomes, and Thespesia populnea using IAA, IBA and NAA was reported. Spectacular increase in the root number was noted in the cuttings of H. fomes and C. iripa treated together with IBA (5000 ppm) and NAA (2500 ppm). The highest number of roots was obtained with IBA (2500 ppm) and NAA (500 ppm) in E. agallocha. B. parviflora and T. populnea responded better to IAA and IBA treatment. The species specific variation in the rooting response to exogenous application of auxins was reflected in the metabolic changes during initiation and development of roots in cuttings. Biochemical analysis showed increase of reducing sugar in the above-girdled tissues at initiation as well as subsequent development of roots which was further enhanced by the use of auxins. Decreases in the total sugar, total carbohydrate and polyphenols and increase in total nitrogen were recorded in the girdled tissues and the high C/N ratio at the initial stage helped in initiation of roots in all the species. Interaction of IBA and NAA promoted starch hydrolysis better than IAA and IBA during root development and subsequently reduced the C/N ratio and increased the protein-nitrogen activity during root development which suggest the auxin influenced mobilization of nitrogen to the rooting zone.
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Abbreviations
- IAA:
-
Indole-3-acetic acid
- IBA:
-
Indole-butyric acid
- NAA:
-
A-naphthalene acetic acid
References
Banerjee LK (1990) Mangroves of Orissa coast and their ecology. Bishen Singh Mohendra Pal Singh, Dehra Dun. p 41
Basak UC, Mahapatro SG and Das P (1994) Effect of plant growth regulators on rooting of Heritiera fomes Buch. Ham Indian J For (In press)
Basu RN and Pal SR (1966) Auxin synergists in the rooting of cuttings. Proc All India Symp Hort. The Royal Agri-Horticultural Society of India, Calcutta, p 45
Basu RN, Roy Choudhury N, Bose TK and Sen PK (1966) Rooting in cuttings of Mangifera indica L. Acta Hortic 24: 61–63
Ghosh SK and Basu RN (1974) Metabolic changes during the regeneration of roots on cuttings. Indian J Exp Biol 12: 166–168
Hartmann HT and Kester DE (1989) Plant Propagation, Principles and Practices. New Delhi: Prentice Hall. 199 p
Jauhari OS and Rahaman SF (1959) Further investigations on rooting in cuttings of sweet lime (Citrus limetoides) Tanaka. Sci Cult 24: 432–434
Nelson N (1944) A photometric adaptation of the Somogyi method for the determination of glucose. J Biol Chem 153: 375–380
Newbold C (1993) Wetland research and survey priorities in the British Isles and their relevance to India. In: Biodiversity Conservation Forest, Wetlands and Deserts, pp 54–67. New Delhi: British High Commission, British Council Division, Tata Energy Research Institute
Patnaik SN and Choudhury BP (1989) Present status and future development of mangrove vegetation in Bitarkanika. In: INDO-US Workshop on Wetlands, Mangroves and Biosphere Reservers, pp 89–95. New Delhi: Ministry of Environment and Forests, Govt of India
Pregle F (1930) Quantitative Organic Microanalysis (Eng. trans. by Fyleman E, 2nd edn.). London: Churchill
Reddy KK, Venkaiah K and Bramaramba B (1994) Rooting of stem cuttings of Avicennia officinalis Linn. and Avicennia alba Bl.: A tool for afforestation of blanks in mangrove forests. Indian For 120: 158–161
Robertson AI, Giddins R and Smith TJ (1990) Seed predation by insects in tropical mangrove forests: Extent and effects on seed viability and the growth of seedlings. Oecologia 83: 213–219
Roy BN, Basu RN and Bose TK (1972) Interaction of auxins with growth-retarding, \s-inhibiting and ethylene producing chemicals in rooting of cuttings. Plant Cell Physiol 13: 1123–1127
Sokal PR and Rohl FJ (1973) Introduction to Biostatistics. San Francisco: Freeman
Somogyi M (1945) A new reagent for determination of sugars. J Biol Chem 160: 61–68
Stoitz LP and Hess CE (1966) The effect of girdling upon root initiation: Auxin and rooting co-factors. Proc Am Soc Hort Sci 89: 744–751
Swain T and Hills WE (1959) The phenolic constituents of Prunus domestica.I. The quantitative analysis of phenolic constituents. J Sci Food Agric 10: 63–68
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Basak, U.C., Das, A.B. & Das, P. Metabolic changes during rooting in stem cuttings of five mangrove species. Plant Growth Regul 17, 141–148 (1995). https://doi.org/10.1007/BF00024174
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DOI: https://doi.org/10.1007/BF00024174