Abstract
Use of15N-depleted fertilizer materials have been primarily limited to fertilizer recovery studies of short duration. The objective of this study was to determine if15N-depleted fertilizer N could be satisfactorily used as a tracer of residual fertilizer N in plant tissue and various soil N fractions through a corn (Zea mays L.) -winter rye (Secale cereale L.) crop rotation. Nitrogen as15N-depleted (NH4)2SO4 was applied at five rates (0, 84, 168, 252, and 336 kg N ha−1) to corn. Immediately following corn harvest a winter rye cover crop treatment was initiated. Residual fertilizer N was easily detected in the soil NO -3 -N fraction following corn harvest (140-d after application). Low levels of exchangeable NH +4 -N (<2.5 mg kg−1) did not permit accurate isotope-ratio analysis. Fertilizer-derived N recovered in the soil total N fraction following corn harvest was detectable in the 0 to 30-cm depth at each N rate and in the 30 to 60 and 60 to 90-cm depths at the 336 kg ha−1 N rate. Atom %15N concentrations in the nonexchangeable NH +4 -N fraction did not differ from the control at each N rate. Nitrogen recovery by the winter rye cover crop reduced residual soil NO -3 -N levels below the 10 kg ha−1 level needed for accurate isotope-ratio analysis. Atom %15N concentrations in the soil total N fraction (approximately one yr after application) were indistinguishable from the control plots below the 168, 252, and 336 kg ha−1 N rate at the 0 to 30, 30 to 60, and 60 to 90-cm depths, respectively. Recovery of residual fertilizer N by the winter rye cover crop was verified by measuring significant decreases in atom %15N concentrations in rye tissue with increasing N rates. The greatest limitation to the use of15N-depleted fertilizer N as a tracer of residual fertilizer N in a corn-rye crop rotation appears to be its detectibility from native soil N in the total N pool.
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References
Broadbent FE and Tyler KB (1957) Nitrification of ammoniacal fertilizers in some California soils. Hilgardia 27: 247–267
Brooks PD, Stark JM, McInteer BB and Preston T (1989) Diffusion method to prepare soil extracts for automated nitrogen-15 analysis. Soil Sci Soc. Am J 53: 1707–1711
Edward AP and Hauck RD (1974) Nitrogen-15 depleted versus nitrogen-15-enriched ammonium sulfate as tracers in nitrogen uptake studies. Soil Sci Soc Am Proc 38: 765–767
Hauck RD and Bremner JM (1976) Use of tracers for soil and fertilizer nitrogen research. Adv. Agron 28: 219–266
Jenkinson DS, Fox RH and Rayner JH (1985) Interactions between fertilizer nitrogen and soil nitrogen-the so-called ‘priming’ effect. J Soil Sci 36: 425–444
Jokela WE and Randall GW (1987) A nitrogen-15 microplot design for measuring plant and soil recovery of fertilizer nitrogen applied to corn. Agron J 79: 322–325
Justice JK and Smith RL (1962) Nitrification of ammonium sulfate in a calcareous soil as influenced by combinations of moisture, temperature and levels of added nitrogen. Soil Sci Soc Am J 51: 366–370
Keeney DR and Nelson DW (1982) Nitrogen inorganic forms. In: AL Page et al (ed) Methods of soil analysis, Part 2, 2nd edition. Agronomy 9: 643–698
Kelley KR, Ditsch DC and Alley MM (1991) Diffusion and automated nitrogen-15 analysis of low-mass ammonium samples. Soil Sci Soc Am J 55: 1016–1020
Kelley KR and Mulvaney RL (1991) Automated nitrogen-15 analysis of plant and soils by Anca/MS and Kjeldahl-Rittenberg technique, p 269. In: Agronomy Abstracts. ASA: Madison, WI.
MacGregor JM, Blake GR and Evans SD (1974) Mineral nitrogen movement into subsoils following continued annual fertilization for corn. Soil Sci Soc Am Proc 38: 110–113
Nommik H and Vahtras (1982) Retention and fixation of ammonium and ammonia in soils. In: FJ Stevenson (ed) Nitrogen in agricultural soils. Agronomy 22: 123–171
Parker JC, Zelazny LW, Sampath S and Harris WG (1979) A critical evaluation of the extension of zero point of charge (ZPC) theory to soil systems. Soil Sci Soc Am J 43: 668–673
Robinson JBD (1975) The soil nitrogen index and its calibration with crop performance to improve fertilizer efficiency on arable soils. Spec Pub No 1 Commonwealth Bureau of Soils, Harpenden, England
Sanchez CA, Blackmer AM, Horton R and Timmons DR (1987) Assessment of errors associated with plot size and lateral movement of nitrogen-15 when studying fertilizer recovery under field conditions. Soil Soc 144: 344–351
SAS Institute (1982) SAS User's guide. SAS Inst, Inc, Cary, NC
Smith SJ, Young LB and Miller GE (1977) Evaluation of soil nitrogen mineralization potentials under modified field conditions. Soil Sci Soc Am J 41: 74–77
Starr JL, Broadbent FE and Stout PR (1974) A comparison of15N-depleted and15N-enrichment fertilizers as tracers. Soil Sci Soc. Am Proc 38: 266–267
Stumpe JM, Vlek PLG, Mughogho SK and Ganry F (1989) Microplot size requirements for measuring balances of fertilizer nitrogen-15 applied to maize. Soil Sci Soc Am J 53: 797–800
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Research partially supported by grants from the National Fertilizer and Environmental Research Center/TVA and the Virginia Division of Soil and Water Conservation.
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Ditsch, D.C., Alley, M.M., Kelley, K.R. et al. Detectability of15N-depleted fertilizer N in soil and plant tissue during a corn-rye crop rotation. Fertilizer Research 31, 355–362 (1992). https://doi.org/10.1007/BF01051287
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DOI: https://doi.org/10.1007/BF01051287