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Proteins of seminal fluid and spermatozoa in the trout (Oncorhynchus mykiss): Partial characterization and variations

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Abstract

The protein composition of seminal fluid, blood serum, sperm plasma membrane and flagellum of rainbow trout were analysed by SDS-polyacrylamide gel electrophoresis. Immunological identity between proteins of the 2 fluids and sperm components was studied using crossed immunoelectrophoresis, rocket immunoelectrophoresis and immunoblotting. Results indicate that many seminal proteins are antigenically-related to serum proteins, proteins of sperm origin are present in seminal fluid in varying amounts, depending on the animals and sampling time, and several serum-like seminal proteins are bound to spermatozoa.

Lipoproteins were isolated from seminal fluid (mean level: 33 μg/ml) and characterized. They were identified as being HDL-like lipoproteins. A possible physiological role is proposed for these seminal lipoproteins.

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Authors and Affiliations

  1. Physiologie des Poissons, INRA, Campus de Beaulieu, 35042, Rennes, France

    M. Loir, C. Labbé, G. Maisse, A. Pinson, G. Boulard, B. Mourot & F. Chambeyron

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  1. M. Loir
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  2. C. Labbé
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  3. G. Maisse
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  4. A. Pinson
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  5. G. Boulard
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  6. B. Mourot
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  7. F. Chambeyron
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Loir, M., Labbé, C., Maisse, G. et al. Proteins of seminal fluid and spermatozoa in the trout (Oncorhynchus mykiss): Partial characterization and variations. Fish Physiol Biochem 8, 485–495 (1990). https://doi.org/10.1007/BF00003405

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