Abstract
Rainbow trout were experimentally infected with the causative agent of bacterial gill disease (BGD) (Flavobacterium branchiophilum) via bath challenge. All fish were cannulated with dorsal aortic catheters, had nasogastric tubes sutured in place for feeding, and were maintained individually, in plexiglass boxes with a flow-through water system. Fish were either fed, or unfed during the trial. Acute changes in blood gas, serum biochemistry and clinical parameters were monitored. By 24h post-challenge, BGD-infected trout that had been fed had significant hypoxemia, hypercapnia, increased blood ammonia, hypoosmolality, hyponatremia, hypochloremia, and increased cough and respiratory rates when compared to control levels. Unfed BGD-infected trout had similar, but less severe blood gas and clinical changes, and no electrolyte disturbances. The BGD-induced hypoxemia is likely exacerbated by increased oxygen demands brought on by feeding. It is not known what association feeding has with the development of low serum ion levels in BGD-infected trout. This is the first study to report the use of fed fish, as opposed to unfed or starved trout, in obtaining blood chemistry values from indisturbed and cannulated animals.
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References cited
Albassam, M., Moore, J. and Sharma, A. 1987. Ultrastructural and clinicopathological studies on the toxicity of cationic acrylamide-based flocculant to rainbow trout. Vet. Pathol. 24: 34–43.
Anacker, R.L. and Ordal, E.J. 1959. Studies on the myxobacterium Chondrococcus columnaris. I Serological typing. J. Bact. 78: 25–32.
Bailly, Y., Dunel-Erb, S., Geffard, M. and Laurent, P. 1989. The vascular and epithelial serotonergic innervation of the actinopterygian gill filament with special reference to the trout, Salmo gairdneri. Cell Tiss. Res. 258: 349–363.
Bergman, H.L., Mattice, J.S. and Brown, D.J.A. 1988. Lake acidification and fisheries project: adult brook trout (Salvelinus fontinalis). Can. J. Fish. Aquat. Sci. 45: 1561–1562.
Booth, C.E., McDonald, D.G., Simons, B.P. and Wood, C.M. 1988. Effects of aluminum and low pH on net ion fluxes and ion balance in the brook trout (Salvelinus fontinalis). Can. J. Fish. Aquat. Sci. 45: 1563–1574.
Boutilier, R.G., Herring, T.A. and Iwama, G.K. 1984. Appendix: physicochemical parameters for use in fish respiratory physiology. In Fish Physiology. Vol. 10A, pp. 403–430. Edited by W.S. Hoar and D.J. Randall. Academic Press, New York.
Bullock, G.L., 1990. Bacterial gill disease of freshwater fishes. Fish Dis. Leaflet 84: 1–4.
Bullock, G.L., Herman, R.L. and Waggy, C. 1991. Hatchery efficacy trials with chloramine-T for control of bacterial gill disease. J. Aquat. An. Health 3: 48–50.
Byrne, P., Speare, D. and Ferguson, H.W. 1989. Effects of a cationic detergent on the gills and blood chemistry of rainbow trout Salmo gairdneri. Dis. Aquat. Org. 6: 185–196.
Byrne, P., Ferguson, H.W., Ostland, V.E. and Lumsden, J.S. 1991. Clinical chemistry of bacterial gill disease in brook trout Salvelinus fontinalis. Dis. Aquat. Orgs. 10: 1–6.
Cameron, J.N. and Heisler, N. 1983. Studies of ammonia in rainbow trout: physico-chemical parameters, acid-base behavior and respiratory clearance. J. Exp. Biol. 105: 107–125.
Daoust, P.-Y. and Ferguson, H.W. 1983. Gill diseases of cultured salmonids in Ontario. Can. J. Comp. Med. 47: 358–362.
Dunel-Erb, S., Bailly, Y. and Laurent, P. 1982. Neuroepithelial cells in fish gill primary lamellae. J. Appl. Physiol. 53: 1342–1353.
Ferguson, H.W. 1989. Systemic Pathology of Fish. Iowa State University Press, Ames.
Ferguson, H.W., Ostland, V.E., Byrne, P. and Lumsden, J.S. 1991. Experimental production of bacterial gill disease in trout by horizontal transmission and by bath challenge. J. Aquat. An. Health 3: 118–123.
Fritsche, R., Thomas, S. and Perry, S.F. 1992. Effects of serotonin on circulation and respiration in the rainbow trout Oncorhynchus mykiss. J. Exp. Biol. 173: 59–73.
From, J. 1980. Chloramine-T for the control of bacterial gill disease. Progr. Fish-Cult. 42: 85–86.
Hauptman, J. and Chaudry, I. H. 1993. Shock: pathophysiology and management of hypovolemia and sepsis. In Textbook of Small Animal Surgery. 2nd Edition, Vol. 1, pp. 1–11. Edited by D. Slatter, W.B. Saunders Company, Toronto.
Laurent, P. 1984. Gill internal morphology. In Fish Physiology. Vol. 10A, pp. 73–183. Edited by W.S Hoar and D.J. Randall. Academic Press, New York.
Lumsden, J.S., Wright, P., Derksen, J., Byrne, P.J. and Ferguson, H.W. 1993. Paralysis in farmed arctic shar (Salvelinus alpinus) associated with ammonia toxicity. Vet. Rec. 133: 422–423.
MacPhee, D.D., Ostland, V.E., Lumsden, J.S. and Ferguson, H.W. 1995. Influence of feeding on the development of bacterial gill disease in rainbow trout (Oncorhynchus mykiss). Dis. Aquat. Org. (In press).
Malte, H. and Weber, R.E. 1989. Gas exchange in fish gills with parallel inhomogeneities. Respir. Physiol. 76: 129–138.
Nilsson, S. 1984. Innervation and pharmacology of the gills. In Fish Physiology. Vol. 10A, pp. 185–227. Edited by W.S Hoar and D.J. Randall. Academic Press, New York.
Ostland, V.E., Ferguson, H.W., Prescott, J.F., Stevenson, R.M.W. and Barker, I.K. 1990. Bacterial gill disease of salmonids; relationship between the severity of gill lesions and bacterial recovery. Dis. Aquat. Org. 9: 5–14.
Ostland, V.E., Lumsden, J.S., MacPhee, D.D. and Ferguson, H.W. 1994. Characteristics of Flavobacterium branchiophilum the cause of salmonid bacterial gill disease in Ontario, Canada. J. Aquat. An. Health 6: 13–26.
Pascoe, P.J. 1987. Emergency care medicine. In Principles and Practice of Veterinary Anesthesia. pp. 558–598. Edited by C.E. Short, Williams & Williams, Baltimore.
Perry, S.F. and Wood, C.M. 1989. Control and coordination of gas transfer in fishes. Can. J. Zool. 67: 2961–2970.
Piiper, J. and Scheid, P. 1984. Model analysis of gas transfer in fish gills. In Fish Physiology. Vol. 10A, pp. 230–262. Edited by W.S Hoar and D.J. Randall. Academic Press, New York.
Randall, D.J. and Wright, P.A. 1989. The interaction between carbon dioxide and ammonia excretion and water pH in fish. Can. J. Zool. 67: 2936–2942.
Schenker, S. and HoyumpaJr., A.M. 1990. Principle complications of liver failure. In Internal Medicine. 3rd Edition, pp. 464–477. Little, Brown and Company (Inc.), Toronto.
Speare, D.J. and Ferguson, H.W. 1989. Clinical features of common gill diseases of salmonids of Ontario. Can. Vet. J. 30: 882–887.
Speare, D.J., Ferguson, H.W., Beamish, F.W.M., Yager, J.A. and Yamashiro, S. 1991. Pathology of bacterial gill disease: ultrastructure of branchial lesions. J. Fish Dis. 14: 1–20.
Speare, D.J., Ferguson, H.W., Beamish, F.W.M., Yager, J.A. and Yamashiro, S. 1991. Pathology of bacterial gill disease: sequential development of lesions during natural outbreaks of disease. J. Fish Dis. 14: 21–32.
Spry, D.J. and Wood, C.M. 1984. Acid-base, plasma ion and blood gas changes in rainbow trout during short term toxic zinc exposure. J. Comp. Physiol. B. 154: 149–158.
Thomas, S., Belaud, A. and Peyraud, C. 1979. Arguments for serotonerigic adjustments in gill blood circulation in fish. IRCS Medical Science: Cardiovascular System; Environmental Biology and Medicine: Experimental Animals; Physiology; Respiratory system 7: 543.
Wakabayashi, H. and Iwado, T. 1985. Changes in glycogen, pyruvate and lactate in rainbow trout with bacterial gill disease. Fish Path. 20: 161–165.
Wakabayashi, H. and Iwado, T. 1985. Effects of bacterial gill disease on the respiratory functions of juvenile rainbow trout. In Fish and Shellfish Pathology. pp. 153–160. Edited by A.E. Ellis. Academic Press, London.
Wolf, K. 1963. Physiological salines for freshwater teleosts. Progr. Fish Cult. 25: 135–140.
Wood, C.M., Playle, R.C., Simons, B.P., Goss, G.G. and McDonald, D.G. 1988. Blood gases, acid-base status, ions, and hematology in adult brook trout (Salvelinus fontinalis) under acid/aluminum exposure. Can. J. Fish. Aquat. Sci. 45: 1575–1586.
Wright, P.A. and Wood, C.M. 1985. An analysis of branchial ammonia excretion in the freshwater rainbow trout: effects of environmental pH change and sodium uptake blockade. J. Exp. Biol. 114: 329–353.
Wright, P.A. and Perry, S.F. 1991. A critical analysis of gas transfer in vivo and in a blood-perfused trout head preparations. Fish Physiol. Biochem. 6: 297–308.
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Byrne, P.J., Ostland, V.E., Lumsden, J.S. et al. Blood chemistry and acid-base balance in rainbow trout Oncorhynchus mykiss with experimentally-induced acute bacterial gill disease. Fish Physiol Biochem 14, 509–518 (1995). https://doi.org/10.1007/BF00004351
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DOI: https://doi.org/10.1007/BF00004351