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The incorporation and metabolism of polyunsaturated fatty acids in phospholipids of cultured cells from chum salmon (Oncorhynchus keta)

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Abstract

The incorporation and metabolism of (n-3) and (n-6) polyunsaturated fatty acids were studied in a cell line derived from chum salmon heart (CHH-1). Supplementing media with 25 μM fatty acid considerably altered the cellular fatty acid composition but did not affect the lipid class composition or cause the appearance of cytoplasmic lipid droplets. CHH-1 cells exhibited considerable Δ-6-desaturase activity but showed no preference between (n-3) and (n-6)PUFA substrates. CHH-1 cells also possess Δ-5-desaturase activity which showed preference towards (n-3)PUFA, but Δ-4-desaturase activity was totally absent. Elongation of 20-carbon PUFA was especially active in CHH-1 cells with 22-carbon PUFA being specifically incorporated into PE and PS lipid classes. The fatty acid composition of PI indicated specific incorporation of 20-carbon PUFA into this lipid class. Supplementation with 22:6(n-3) generated fatty acid compositions more closely resembling those of intact salmonid hearts. Substantial chain shortening of 22:6(n-3) to 20:5(n-3) occurred.

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Abbreviations

BHT:

butylated hydroxytoluene

BSA:

bovine serum albumin

CL:

cardiolipin

FCS:

fetal calf serum

PA:

phosphatidic acid

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PI:

phosphatidylinositol

PS:

phosphatidylserine

PUFA:

polyunsaturated fatty acid

SM:

sphingomyelin

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Authors and Affiliations

  1. N.E.R.C. Unit of Aquatic Biochemistry, School of Natural Sciences, University of Stirling, Stirling, FK9 4LA, Scotland

    J. Gordon Bell & John R. Sargent

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  1. J. Gordon Bell
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  2. John R. Sargent
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Gordon Bell, J., Sargent, J.R. The incorporation and metabolism of polyunsaturated fatty acids in phospholipids of cultured cells from chum salmon (Oncorhynchus keta). Fish Physiol Biochem 10, 99–109 (1992). https://doi.org/10.1007/BF00004521

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  • DOI: https://doi.org/10.1007/BF00004521

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