Summary
Ten cultivars of oats (Avena sativa L.) were investigated for their isozymes of esterase (E), leucine aminopeptidase (LAP), and anodal and cathodal peroxidase (APX and CPX).
From repeated observations, a typical zymogram was prepared for each enzyme system, and the frequencies of the isozyme bands were scored for each variety separately. The results showed that: (1) most of the isozyme bands are very stable in their appearance on the gel: (2) each variety has a characteristic isozyme pattern; (3) 7 out of 10 oat cultivars showed no intravarietal isozyme variation. Some variation was found for the varieties Mesa, Rapida and Curt. This overall high level of homozygosity and homogeneity of these varieties for the isozyme bands makes this technique useful for varietal identification.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Almgard, G. & T. Norman, 1970. Biochemical technique as an aid to distinguishing some cultivars of barley and oats. Agric. Hort. Genet. 28: 117–123.
Ayala, G. J., C. A. Maurao, S. Perez-Salas, R. Richmond & Th. Dobzhansky, 1970. Enzyme variability in the Drosophila willistoni group. I. Genetic differentiation among sibling species. Proc. natn. Acad. Sci. USA. 67: 225–232.
Barber, H. N., C. J. Driscoll, R. M. Long & R. S. Vickery, 1968. Protein genetics of wheat among homeologous relationships of chromosomes. Nature, Lond. 218: 450–452.
Baum, B. R., 1969. Pedigree and other basic data of cultivars of oats. Research Branch, Canada Department of Agriculture.
Brown, A. H. D. & R. W. Allard, 1969. The inheritance of isozyme differences among the inbred parents of a reciprocal recurrent selection population of corn. Crop Sci. 9: 72–75.
Chen, C. H. & W. Bushuk, 1970. Nature of proteins in Triticale and its parental species. III. A comparison of their electrophoretic patterns. Can. J. Pl. Sci. 50: 25–30.
Harris, H., 1971. Protein polymorphisms in man. Can. J. Genet. Cytol. 13:381–396.
Hubby, J. L. & R. C. Lewontin, 1966. A molecular approach to the study of genic heterozygosity in natural populations. I. The nature of alleles at different loci in Drosophila pseudoobscura. Genetics 54:577–594.
Hubby, J. L. & L. H. Throckmorton, 1968. Protein differences in Drosophila. IV. A study of sibling species. Am. Nat. 102: 193–205.
Johnson, B. L., 1969. The protein electrophoresis approach to species relationships in wheat. In R. Bogart (Ed.), Genetics lectures, Vol. 1: 19–44, Oregon State University Press, Oregon.
Johnson, B. L., D. Barnhart & O. Hall, 1967. Analysis of genome and species relationships in the polyploid wheats by protein electrophoresis. Am. J. Bot. 54: 1089–1098.
Johnson, F. M., C. G. Kanapi, R. H. Richardson, M. R. Wheeler & W. S. Stone, 1966. An operational classification of Drosophila esterases for species comparisons. Univ. Texas Publ. 6615: 517–532.
Kahler, A. L. & R. W. Allard, 1970. Genetics of isozyme variants in barley. I. Esterase. Crop Sci. 10: 444–448.
Kristjansson, F. K., 1963. Genetic control of two prealbumins in pigs. Genetics 48: 1059–1063.
Marshall, D. R. & R. W. Allard, 1969. The gentics of electrophoretic variants in Avena. I. The esterase E4, E9, E10, phosphatase P5, and anodal perodixase APX5 loci in A. barbata. J. Hered. 60: 17–19.
Marshall, D. R. & R. W. Allard, 1970. Maintenance of isozyme polymorphisms in natural populations of Avena barbata. Genetics 66: 393–399.
Menke, J. F., R. S. Singh, C. O. Qualset & S. K. Jain, 1973. Protein electrophoresis aids wheat cereal identification. Calif. Agric. (in press).
Murray, B. E., I. L. Craig, & T. Rajhathy, 1970. A protein electrophoretic study of three amphiploids and eight species in Avena. Can. J. Genet. Cytol. 12: 651–665.
Nair, P. S., D. Brncic, & K. Kojima, 1971. Isozyme variations and evolutionary relationships in the Mesophragmatica species group of Drosophila. Univ. Texas Publ. 7103: 17–28.
Prakash, S., R. C. Lewontin, & J. L. Hubby, 1969. A molecular approach to the study of genic heterozygosity in natural populations. IV. Patterns of genic variation in central, marginal and isolated populations of Drosophila pseudoobscura. Genetics 61: 841–858.
Scandalios, J. G., 1964. Tissue specific isozyme variations in maize. J. Hered. 55: 281–285.
Scandalios, J. G., 1969. Genetic control of multiple molecular forms of enzymes in plants: A review. Biochem. Genet. 3: 37–79.
Selander, R. K., M. H. Smith, S. Y. Yang, W. E. Johnson & J. B. Gentry, 1971. Biochemical polymorphisms and systematics in the genus Peromyscus. I. Variation in the old field mouse. Univ. Texas Publ. 7103: 49–90.
Shaw, C. R. & A. L. Koen, 1968. Starch gel zone electrophoresis of enzymes. In: Ivor Smith (Ed.), Chromatographic and electrophoretic techniques, Vol. 2: 325–364. Interscience Publishers, New York.
Smith, H. H., D. E. Hamill, E. A. Weaver & K. H. Thompson, 1970. Multiple molecular forms of peroxidases and esterases among Nicotiana species and amphiploids. J. Hered. 61: 203–212.
Suneson, C. A., 1967. Registration of Rapida oats. Crop Sci. 7: 168.
Suneson, C. A., 1969. Registration of Montezuma oats. Crop Sci. 9: 848–849.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Singh, R.S., Jain, S.K. & Qualset, C.O. Protein electrophoresis as an aid to oat variety identification. Euphytica 22, 98–105 (1973). https://doi.org/10.1007/BF00021560
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00021560