Abstract
Gliadin proteins extracted from fifteen Chinese and Yugoslav winter wheat cultivars were fractionated using a new separation technique – Capillary Zone Electrophoresis (CZE). Different CZE conditions were defined to optimize resolution and reproducibility of gliadin separations. Excellent resolution and high reproducibility of gliadin CZE patterns were obtained by using 47 cm length, 50 μm i.d. capillaries at 15 kV and 30° C in sodium borate buffer system with acetonitrile (ACN) and sodium dodecyl sulfate. By using these CZE conditions, gliadin proteins from each cultivar were easily separated into more than 35 components. This resolution is generally superior to that of one- and two-dimensional electrophoresis and RH-HPLC. Analysis of reproducibility of gliadin CZE patterns from Chinese cultivar ‘Lumai 6’ showed that the average relative standard deviation (RSD) for peak migration times and heights was 0.21% and 4.06%, respectively. Gliadin electrophoregrams of all cultivars studied showed clear qualitative and quantitative differences, including presence or absence of some major peak, migration times and heights of peaks. Specifically, some closely related cultivars that were not differentiable by A-PAGE, were readily differentiated by CZE. In addition, winter wheat cultivars from China and Yugoslavia showed greater differences in gliadin compositions revealed by CZE.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bietz, J.A., 1983. Reversed-phase high-performance liquid chromatography of cereal endosperm proteins. J Chromatrogr 255: 219–238.
Bietz, J.A., 1994. Fractionation of wheat gluten proteins by capillary electrophoresis. In: Gluten Proteins 1993. Association of Cereal Research, Detmold, Germany, pp. 404–4113.
Bietz, J.A. & E. Schmalzried, 1995. Capillary electrophoresis of wheat gliadin: Initial studies and application to varietal identification. Lebensm-Wiss u-Technol 28: 174–184.
Burnouf, T. & J.A. Bietz, 1987. Identification of wheat cultivars and prediction of quality by reversed-phase high-performance liquid chromatographic analysis of endosperm storage proteins. Seed Sci Technol 15: 79–99.
Draper, S.R., 1987. ISTA Variety Committee Report of theWorking Group for Biochemical Tests for Cultivar Identification 1983–1986. Seed Sci Technol 15: 431–434.
Huebner, F.R. & J.A. Bietz, 1994. RP-HPLC for varietal identification in cereals and legumes, Wheat. In: J.E. Kruger & J.A. Bietz (Eds), HPLC of Cereal and Legume Proteins. St. Paul, MN: American Association of Cereal Chemists, pp. 97–120.
Jones, B.I., G.L. Lookhart, S.B. Hall & K.F. Finney, 1982. Identification of wheat cultivars by gliadin electrophoresis: electrophoregrams of the 88 wheat cultivars most commonly grown in the United States in 1979. Cereal Chem 59: 181–188.
Lafiandra, D. & D.D. Kasarda, 1985. One-and two-dimensional (two-pH) polyacrylamide gel electrophoresis in a single gel: separation of wheat proteins. Cereal Chem 62: 314–319.
Landers, J.P., 1991. High performance capillary electrophoresis of biomolecules. Bio Essays 13: 253–258.
Lookhart, G.L. & S.R. Bean, 1995a. A fast method for wheat cultivar differentiation using capillary zone electrophoresis. Cereal Chem 72: 42–47.
Lookhart, G.L. & S.R. Bean, 1995b. Rapid differentiation of oat cultivars and of rice cultivars by capillary zone electrophoresis. Cereal Chem 72: 312–316.
Lookhart, G.L. & S.R. Bean, 1995c. Separation and characterization of wheat protein fractions by high-performance capillary electrophoresis. Cereal Chem 72: 527–532.
Lookhart, G.L. & S.R. Bean, 1996. Improvement in cereal protein separations by capillary electrophoresis: Resolution and reproducibility. Cereal Chem 73: 81–87.
Marchylo, B.A., 1987. Identification of Canadian and American wheat cultivars by SDS gradient PAGE analysis of gliadin and glutenin subunits. Can J Plant Sci 67: 945–952.
Metakovsky, E.V. & A. Yu. Novoselskaya, 1991. Gliadin allele identification in common wheat, I. Methodological aspects of the analysis of gliadin patterns by one-dimensional polyacrylamide gel electrophoresis. J Genet & Breed 45: 317–324.
Smith, J.S.C., 1986. Biochemical fingerprints of cultivars using reversed-phase high-performance liquid chromatography and isozyme electrophoresis: a review. Seed Sci Technol 14: 753–768.
Werner, W.E., J.E. Wiktorowicz & D.D. Kasarda, 1994. Wheat varietal identification by capillary electrophoresis of gliadins and high molecular weight glutenin subunits. Cereal Chem 71: 397–402.
Yan, Y., B. Jovanovic, S. Prodanovic & D. Zoric, 1994. Application of electrophoresis for gliadin characterisation and varietal identification in wheat. J Sci Agric Res 55.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yan, Y., Surlan-Momirovic, G., Prodanovic, S. et al. Capillary zone electrophoresis analysis of gliadin proteins from Chinese and Yugoslav winter wheat cultivars. Euphytica 105, 197–204 (1999). https://doi.org/10.1023/A:1003431602558
Issue Date:
DOI: https://doi.org/10.1023/A:1003431602558