Abstract
The novel application of scanning electron microscopy to study whole-mount surface-spread synaptonemal complex complements of rye (Secale cereale) and rat (Rattus norvegicus) is described. Scanning electron microscopy is able to resolve the third dimension in such preparations and improve the tracing of the continuity of lateral elements without losing information that could be obtained by conventional transmission electron microscopy. This improvement is likely to benefit detailed studies of chromosome synapsis and karyology, and may provide a means of circumventing technical obstacles inhibiting the use of surface-spreads as substrates forin situ hybridization under the electron microscope.
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References
Albini SM, Jones GH (1984) Synaptonemal complex-associated centromeres and recombinant nodules in plant meiocytes prepared by an improved surface-spreading technique.Exp Cell Res 155: 588–592.
Albini SM, Schwarzacher T (1992)In situ localisation of two repetitive DNA sequences to surface-spread pachytene chromosomes of rye.Genome 35: 551–559.
Albini SM, Jones GH, Wallace BMN (1984) A method for preparing two-dimensional surface-spreads of synaptonemal complexes from plant meiocytes for light and electron microscopy.Exp Cell Res 152: 280–285.
Barlow AL (1992)A Study of the Synaptonemal Complex Using Scanning Electron Microscopy. M.Sc. Thesis, University of Wales, Aberystwyth.
Bloom SE, Goodpasture C (1976) An improved technique for selective silver staining of nucleolar organizer regions in human chromosomes.Hum Genet 34: 199–206.
Christenhuss R, Buchner T, Pfeifer RA (1967) Visualisation of human somatic chromosomes by scanning electron microscopy.Nature 216: 379–380.
Counce SJ, Meyer GF (1973) Differentiation of the synaptonemal complex and the kinetochore inLocusta spermatocytes studied by whole mount electron microscopy.Chromosoma 44: 231–253.
Dille JE, Bittel, DC, Ross K, Gustafson JP (1990) Preparing plant chromosomes for scanning electron microscopy.Genome 33: 333–339.
Fawcett DW (1956) The fine structure of chromosomes in the meiotic prophase of vertebrate spermatocytes.J Biophys Biochem Cytol 2: 403–406.
Gillies CB (1984) The synaptonemal complex in higher plants.CRC Crit Rev Plant Sci 2: 81–116.
Harrison JH, Allen, TD, Britch M, Harris R (1982) High resolution scanning electron microscopy of human metaphase chromosomes.J Cell Sci 56: 409–422.
Hasenkampf CA (1989) A method for producing synaptonemal complex complements in lily and mouse.J Hered 80: 197–202.
Hasenkampf CA (1991)In situ hybridization ofLilium whole mount synaptonemal complex chromosomal preparations.Biotech Histochem 66: 210–215.
Howell WM, Black DA (1980) Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method.Experientia 36: 1014–1015.
Loidl J (1989) Effects of elevated temperature on meiotic chromosome synapsis inAllium ursinum.Chromosoma 97: 449–458.
Moens PB, Pearlman RE (1989) Satellite DNA I in chromatin loops of rat pachytene chromosomes and spermatids.Chromosoma 98: 287–294.
Moens PB, Pearlman RE (1990a) Telomere and centromere DNA are associated with the cores of meiotic prophase chromosomes.Chromosoma 100: 8–14.
Moens PB, Pearlman RE (1990b)In situ DNA sequence mapping with surface-spread mouse pachytene chromosomes.Cytogenet Cell Genet 53: 219–220.
Moses MJ (1956) Chromosomal structures in crayfish spermatocytes.J Biophys Biochem Cytol 2: 215–217.
Rasmussen SW (1986) Initiation of synapsis and interlocking of chromosomes during zygotene inBombyx spermatocytes.Carlsberg Res Commun 51: 401–432.
Scherthan H, Loidl J, Schuster T, Schweizer D (1992) Meiotic chromosome condensation and pairing inSaccharomyces cerevisiae studied by chromosome painting.Chromosoma 101: 590–595.
Schubert I, Dolezel J, Houben A, Scherthan H, Wanner G (1993) Refined examination of plant metaphase chromosome structure at different levels made feasible by new isolation methods.Chromosoma 102: 96–101.
Schwarzacher T, Heslop-Harrison JS (1991)In situ hybridisation to plant telomeres using synthetic oligomers.Genome 34: 317–323.
Stack SM (1982) Two-dimensional spreads of synaptonemal complexes from solanaceous plants I. The technique.Stain Tech 57: 265–272.
Von Wettstein D, Rasmussen SW, Holm PB (1984) The synaptonemal complex in genetic segregation.Annu Rev Genet 18: 331–413.
Wanner G, Formanek H, Martin R, Herrmann RG (1991) High resolution scanning electron microscopy of plant chromosomes.Chromosoma 100: 103–109.
Westergaard M, Von Wettstein D (1972) The synaptonemal complex.Annu Rev Genet 6: 71–110.
Whelan EDP, Haggis GH, Ford EJ, Dronzek B (1974) Scanning electron microscopy of meiotic chromosomes of plantsin situ.Can J Bot 52: 1438–1440.
Whelan EDP, Kokko EG (1992) Scanning electron microscopy of chromosomes of common wheat.Genome 35: 166–169.
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Barlow, A.L., Jenkins, G. & ap Gwynn, I. Scanning electron microscopy of synaptonemal complexes. Chromosome Res 1, 9–13 (1993). https://doi.org/10.1007/BF00710602
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DOI: https://doi.org/10.1007/BF00710602