Abstract
This paper describes NMR measurements of 15N–15N and 1H–15N scalar couplings across hydrogen bonds in Watson–Crick base pairs, h2 J NN and h1 J HN, in a 17 kDa Antennapedia homeodomain–DNA complex. A new NMR experiment is introduced which relies on zero-quantum coherence-based transverse relaxation-optimized spectroscopy (ZQ-TROSY) and enables measurements of h1 J HN couplings in larger molecules. The h2 J NN and h1 J HN couplings open a new avenue for comparative studies of DNA duplexes and other forms of nucleic acids free in solution and in complexes with proteins, drugs or possibly other classes of compounds.
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Pervushin, K., Fernández, C., Riek, R. et al. Determination of h2JNN and h1JHN coupling constants across Watson–Crick base pairs in the Antennapedia homeodomain–DNA complex using TROSY. J Biomol NMR 16, 39–46 (2000). https://doi.org/10.1023/A:1008367405025
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DOI: https://doi.org/10.1023/A:1008367405025