Summary
The expression of the intermediate filament protein, vimentin, was studied in skeletal muscle during a cycle of degeneration and regeneration. Venom from the Australian tiger snake,Notechis scutatus scutatus, was used to initiate the breakdown of the soleus muscle of young, mature ratsin vivo. Cryosections and Western blots of muscle samples were labelled using antibodies to vimentin, and examined at fixed time points after venom injection. Vimentin was absent in control adult muscle fibres, but was identified in activated satellite cells 12 h after venom assault. The amount of this protein rose during the early stages of regeneration, reaching its peak at 2–3 days. At this time, the expression of muscle-specific intermediate filament protein, desmin, began. As the abundance of desmin increased with the maturation of the regenerating myofibres, the abundance of vimentin declined until it was no longer detectable in mature regenerated fibres. It is suggested that vimentin plays an important role during satellite cell activation in the early stages of regeneration, and that the expression of vimentin may act as a stimulus for the expression of desmin at later stages of regeneration.
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Bennett,G. S.,Fellini,S. A.,Toyama,Y. &Holtzer,H. (1979) Redistribution of intermediate filaments subunits during skeletal myogenesis and maturationin vitro.J. Cell Biol. 82, 577–84.
Bornemann,A. &Schmalbruch,H. (1992) Desmin and vimentin in regenerating muscles.Muscle Nerve 15, 14–20.
Cullen,M. J. &Fulthorpe,J. J. (1982) Phagocytosis of the A band following Z line and I band loss. Its significance in skeletal muscle breakdown.J. Pathol. 138, 129–43.
Cullen,M. J.,Hudgson,P. &Mastaglia,F. L. (1988) Ultrastructural studies of diseased muscle. InDisorders of Voluntary Muscle, 5th edn. (edited byWalton,J. N.), pp. 284–344. Edinburgh: Churchill Livingstone.
Fujimoto,T.,Tokuyasu,K. T. &Singer,S. J. (1987) Direct morphological demonstration of the coexistence of vimentin and desmin in the same intermediate filaments of vascular smooth muscle cells.J. Submicrosc. Cytol. 19, 1–9.
Gallanti,A.,Prelle,A.,Moggio,M.,Ciscato,P.,Checcarelli,N.,Sciacco,N.,Comini,A. &Scarlato,G. (1992) Desmin and vimentin as markers of regeneration in muscle diseases.Acta Neuropathol. 85, 88–92.
Gard,D. L. &Lazarides,E. (1980) The synthesis and distribution of desmin and vimentin during myogenesisin vitro.Cell 19, 262–75.
Granger,B. L. &Lazarides,E. (1979) Desmin and vimentin coexist at the periphery of the myofibril Z disc.Cell 18, 1053–63.
Harris,J. B. &Cullen,M. J. (1990) Muscle necrosis caused by snake venoms and toxins.Electron Microsc. Rev. 3, 183–211.
Harris,J. B. &Johnson,M. A. (1978) Further observations on the pathological responses of rat skeletal muscle to toxins isolated from the venom of the Australian tiger snake,Notechis scutatus scutatus.Clin. Exp. Pharmacol. Physiol. 5, 587–600.
Harris,J. B.,Johnson,M. A. &Karlsson,E. (1975) Pathological responses of rat skeletal muscle to a single subcutaneous injection of a toxin isolated from the venom of the Australian tiger snake,Notechis scutatus scutatus.Clin. Exp. Pharmacol. Physiol. 2, 383–404.
Klein-Ogus,C. &Harris,J. B. (1983) Preliminary observations of satellite cells in undamaged fibres of the rat soleus muscle assaulted by a snake-venom toxin.Cell Tiss. Res. 230, 671–6.
Lazarides,E. (1980) Intermediate filaments as mechanical integrators of space.Nature 283, 249–56.
Maltin,C. A.,Harris,J. B. &Cullen,M. J. (1983) Regeneration of mammalian skeletal muscle following the injection of the snake-venom toxin, taipoxin.Cell Tiss. Res. 232, 565–77.
Nicholson,L. V. B.,Davison,K.,Falkous,G.,Harwood,C.,O'Donnell,E.,Slater,C. R. &Harris,J. B. (1989) Dystrophin in skeletal muscle: I. Western blot analysis using a monoclonal antibody.J. Neurol. Sci. 94, 125–36.
Osborn,M.,Caselitz,J.,Puschel,K. &Weber,K. (1987) Intermediate filament expression in human vascular smooth muscle and in arteriosclerotic plaques.Virchows Arch. A. 411, 449–58.
Sarnat,H. B. (1992) Vimentin and desmin in maturing skeletal muscle and developmental myopathies.Neurology 42, 1616–24.
Schultheiss,T.,Lin,Z.,Ishikawa,H.,Zamir,I.,Stoeckert,C. J. &Holtzer,H. (1991) Desmin/vimentin intermediate filaments are dispensable for many aspects of myogenesis.J. Cell Biol. 114, 953–66.
Steinert,P. M.,Jones,J. C. R. &Goldman,R. D. (1984) Intermediate filaments.J. Cell Biol. 99, 22s-27s.
Thornell,L.-E.,Eriksson,A. &Edstrom,L. (1983) Intermediate filaments in human myopathies. InCell and Muscle Motility, 4th edn. (edited byDowben,R. M. &Shay,J. W.), pp. 85–136. New York: Plenum Press.
Tokuyasu,K. T.,Maher,P. A. &Singer,S. J. (1984) Distribution of desmin and vimentin in developing chick myotubesin vivo. I. Immunofluorescence study.J. Cell Biol. 98, 1961–72.
Tokuyasu,K. T.,Maher,P. A. &Singer,S. J. (1985) Distributions of desmin and vimentin in developing chick myotubesin vivo. II. Immunoelectron microscopic study.J. Cell Biol. 100, 1157–66.
Traub,P. (1985)Intermediate Filaments. A Review. pp. 1–266. Berlin: Springer-Verlag.
Vater,R.,Cullen,M. J. &Harris,J. B. (1992a) The fate of dystrophin during the degeneration and regeneration of the soleus muscle of the rat.Acta Neuropathol. 83, 140–8.
Vater,R.,Cullen,M. J. &Harris,J. B. (1992b) The fate of desmin and titin during the degeneration and regeneration of the soleus muscle of the rat.Acta Neuropathol. 84, 278–88.
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Vater, R., Cullen, M.J. & Harris, J.B. The expression of vimentin in satellite cells of regenerating skeletal musclein vivo . Histochem J 26, 916–928 (1994). https://doi.org/10.1007/BF02388568
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DOI: https://doi.org/10.1007/BF02388568