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Production ofBeauveria bassiana [Deuteromycotina: Hyphomycetes] in different liquid media and subsequent conidiation of dry mycelium

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Abstract

Mycelium ofBeauveria bassiana can be grown in liquid culture, filtered, and the mycelium dried. After rehydration the mycelium sporulates.

Two carbohydrate sources (sucrose and maltose), and one nitrogen/vitamin source (yeast extract) were tested for mycelium growth and subsequent conidial production. Maximum mycelium growth (12.31 mg/ml), in liquid culture, was in the sucrose (3.5%)/yeast extract (3.5%) medium, but mycelium from a maltose (2%)/yeast extract (0.75%) medium produced the maximum of 4.62×106 conidia/mg dry mycelium after incubation in moist Petri dishes.

Using the data on mycelium yield (in liquid culture) and conidial production (by dry mycelium) it is calculated that the sucrose (3.5%)/yeast extract (3.5%) and the maltose (2%)/yeast extract (0.75%) media produce most conidia per media volume (an equivalent of 3.52–3.72×107 conidia/ml).

Résumé

Le mycélium deBeauveria bassiana peut être développé en milieu liquide, filtré et le mycélium séché. Après réhydratation, le mycélium sporule.

Deux sources d'hydrates de carbone (sucrose et maltose) et une source azote/vitamine (extrait de levure) furent essayées pour la croissance du mycélium et la production consécutive de conidies. La croissance maximale du mycélium (12,31 mg/ml) en culture liquide, intervenait dans un milieu: sucrose (3,5%)/extrait de levure (3,5%), mais le mycélium issu d'un milieu maltose (2%)/extrait de levure (0.75%) produisait le maximum de 4,62×106 conidies/mg de mycélium sec après incubation en boîtes humides de Pétri.

En utilisant les données sur la production de mycélium (en culture liquide) et sur celle de conidies (par le mycélium sec), on a calculé que les milieux sucrose (3,5%)/extrait de levure (3,5%) et maltose (2%)/extrait de levure (0,75%) produisaient la plus grande quantité de conidies par volume de milieu (l'équivalent de 3,52–3,72×107 conidies/ml).

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Authors and Affiliations

  1. Insect Pathology Resource Center, Boyce Thompson Institute for Plant Research at Cornell, Tower Road, 14853, Ithaca, New York, U.S.A.

    M. C. Rombach & D. W. Roberts

  2. Entomology Department, International Rice Research Institute, P.O. Box 933, Manila, the Philippines

    R. M. Aguda

Authors
  1. M. C. Rombach
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  2. R. M. Aguda
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  3. D. W. Roberts
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Rombach, M.C., Aguda, R.M. & Roberts, D.W. Production ofBeauveria bassiana [Deuteromycotina: Hyphomycetes] in different liquid media and subsequent conidiation of dry mycelium. Entomophaga 33, 315–324 (1988). https://doi.org/10.1007/BF02372621

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  • DOI: https://doi.org/10.1007/BF02372621

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