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Mapping the distribution of 3-hydroxylipins in the Mucorales using immunofluorescence microscopy

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Abstract

The distribution of endogenous 3-hydroxylipins (3-OH oxylipins) in representatives of the Mucorales was mapped using immunofluorescence microscopy. Strains of each of the following genera were examined: Absidia, Actinomucor, Cunninghamella, Mortierella (subgenus Micromucor), Mortierella (subgenus Mortierella), Mucor and Rhizomucor. Immunofluorescence microscopy was carried out using an antibody that was raised against 3R-hydroxy-5Z,8Z,11Z,14Z-eicosatetraenoic acid (3R-HETE), which cross-reacts with other 3-OH oxylipins. Subsequently, the occurrence and distribution of the antibody on the various reproductive stages of each fungus was noted. In Absidia, Actinomucor, Mortierella (subgenus Micromucor), Mucor and Rhizomucor, 3-OH oxylipins were found to be associated with the columellae and/or wall of the sporangium. In the representative of Cunninghamella, the 3-OH oxylipins were associated with the single-spored sporangiola. No 3-OH oxylipins were detected in the strains representing Mortierella (subgenus Mortierella).

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Correspondence to J.L.F. Kock.

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Strauss, T., Botha, A., Kock, J. et al. Mapping the distribution of 3-hydroxylipins in the Mucorales using immunofluorescence microscopy. Antonie Van Leeuwenhoek 78, 39–42 (2000). https://doi.org/10.1023/A:1002757628619

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  • DOI: https://doi.org/10.1023/A:1002757628619

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