Abstract
The immunoaccessibility of the H8 antigen was examined as was the functionality of H8 specific monoclonal antibody (McAb 10). Immunoaccessibility of H8 varied among individual gonococci grown in vitro; the overall degree of variability was strain dependent. The H8 antigen was detected on the surface of most but not all gonococci found in urine sediment from an infected volunteer. The H8 specific McAb 10 was not bactericidal for strains of serum resistant gonococci; in the serum sensitive strain, an increase of approximately .5 logs above the bactericidal activity of antibody depleted normal human serum was observed. It is possible that the apparent paradox of the common, immunogenic, antigenic surface constituent H8 may be resolved in part by variation in immunoaccessibility within and among gonococcal strains.
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Black, J. R., W. J. Black & J. G. Cannon (1985) Neisserial antigen H8 is immunogenic in patients with disseminated gonococcal and meningococcal infections. J. Infect. Dis. 151: 650–657
Black, W. J. & J. G. Cannon (1985) Cloning of the gene for the common pathogenic Neisseria H8 antigen from Neisseria gonorrhoeae. Infect. Immun. 47: 322–325
Cannon, J. G., W. J. Black, I. Nachamkin & P. W. Stewart (1984) Monoclonal antibody that recognizes an outer membrane antigen common to the pathogenic Neisseria species but not to most nonpathogenic Neisseria species. Infect. Immun. 43: 994–999
Hildebrandt, J. F., L. W. Mayer, S. P. Wang & T. M. Buchanan (1978) Neisseria gonorrhoeae acquire a new principal outer-membrane protein when transformed to resistance to serum bactericidal activity. Infect. Immun. 20: 267–273
Hitchcock, P. J. & T. M. Brown (1983) Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J. Bacteriol. 154: 269–277
Hitchcock, P. J., S. F. Hayes, L. W. Mayer, W. M. Shafer & S. L. Tessier (1985). Analyses of gonococcal H8 antigen. Surface location, inter- and intrastrain electrophoretic heterogeneity, and unusual two-dimensional electrophoretic characteristics. J. Exp. Med. 162: 2017–2034
James, J. F. & J. Swanson (1978) Studies on gonococcus infection. XIII. Occurrence of color/opacity colonial variants in clinical cultures. Infect. Immun. 19: 332–340
Joiner, K. A., K. A. Warren, E. J. Brown, J. Swanson & M. M. Frank (1983) Studies on the mechanism of bacterial resistance to complement-mediated killing. IV. C5B-9 forms high molecular weight complexes with bacterial outer membrane constituents on serum-resistant but not on serum-sensitive Neisseria gonorrhoeae. J. Immunol. 131: 1443–1451
Robinson, E. N.Jr, Z. A. McGee, T. M. Buchanan, M. S. Blake & P. J. Hitchcock (1987) Probing the surface of Neisseria gonorrhoeae: simultaneous localization of protein I and H8 antigens. Infect. Immun. 55: 1190–1197
Robinson, E. N.Jr., Z. A. McGee, J. Kaplan, M. E. Hammond, J. K. Larson, T. M. Buchanan & G. K. Schoolnik (1984) Ultrastructural localization of specific gonococcal macromolecules with antibody-gold sphere immunological probes. Infect. Immun. 46: 361–366
Strittmatter, W. G. & P. J. Hitchcock (1986) Isolation and preliminary characterization of gonococcal H8 antigen. J. Exp. Med. 164: 2038–2048
Swanson, J. & O. Barrera (1983) Immunological characteristics of gonococcal outer membrane protein II assessed by immunoprecipitation, immunoblotting, and coagglutination. J. Exp. Med. 157: 1405–1420
Swanson, J., S. Bergstrom, O. Barrera, K. Robbins & D. Corwin (1985) Pilus gonococcal variants. Evidence for multiple forms of piliation control. J. Exp. Med. 162: 729–744
Wallace, R., F. E. Ashton, A. Ryan & B. B. Diena (1978) The lipopolysaccharide (R type) as a common antigen of Neisseria gonorrhoeae. II. Use of hen antiserum to gonococcal lipopolysaccharide in a rapid slide test for the identification of N. gonorrhoeae from primary isolates and secondary cultures. Can. J. Microbiol. 24: 124–129
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Hitchcock, P.J., Boslego, J., Joiner, K.A. et al. Analyses of the immunoaccessibility of H8 antigen and the functionality of H8 specific monoclonal antibody 10. Antonie van Leeuwenhoek 53, 509–518 (1987). https://doi.org/10.1007/BF00415510
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DOI: https://doi.org/10.1007/BF00415510