Abstract
The adsorption of the defective phage PBS Z of Bacillus subtilis has previously been assumed to proceed in two steps, a reversible adsorption of extended phages followed by contraction of the adsorbed particles (Steensma, 1981a). This model, also used for other phages, explained the biphasic character of the adsorption curve, but a discrepancy was found between the calculated and observed concentrations of adsorbed, extended phages. Computer simulations indicated that this might be caused by inhomogeneity of the phage preparations with respect to their adsorption properties and that in that case other models would also fit the experimental data. Discrimination between the models was not possible on the basis of the available information on PBS Z and it was therefore concluded that the values reported previously for the rate constants (Steensma, 1981a) should be used with caution.
This is a preview of subscription content, log in via an institution to check access.
References
Christensen, J. R. 1965. The kinetics of reversible and irreversible attachment of bacteriophage T1. — Virology 26: 727–737.
Crawford, J. T. and Goldberg, E. B. 1980. The function of tail fibers in triggering baseplate expansion of bacteriophage T4. — J. Mol. Biol. 139: 679–690.
Garen, A. 1954. Thermodynamic and kinetic studies on the attachment of T1 bacteriophage to bacteria. — Biochim. Biophys. Acta 14: 163–172.
Garen, A. and Puck, T. T. 1951. The first two steps of the invasion of host cells by bacterial viruses II. — J. Exp. Med. 94: 177–189.
Haas, M. and Yoshikawa, H. 1969. Defective bacteriophage PBS H in Bacillus subtilis. I. Induction, purification and physical properties of the bacteriophage and its deoxyribonucleic acid. — J. Virol. 3: 233–247.
Heller, K. and Braun, V. 1979. Accelerated adsorption of bacteriophage T5 to Escherichia coli F, resulting from reversible tail fiber-lipopolysaccharide binding. — J. Bacteriol. 139: 32–38.
Marquardt, D. W. 1963. An algorithm for least squares estimation of non-linear parameters. —J. Soc. Ind. Appl. Math. 11: 431–441.
Puck, T. T., Garen, A. and Cline, J. 1951. The mechanism of virus attachment to host cells. I. The role of ions in the primary reaction. — J. Exp. Med. 93: 65–88.
Schwartz, M. 1975. Reversible interaction between coliphage lambda and its receptor protein. — J. Mol. Biol. 99: 185–201.
Schwartz, M. 1976. The adsorption of coliphage lambda to its host: effect of variations in the surface density of receptor and in phage-receptor affinity. — J. Mol. Biol. 103: 521–536.
Steensma, H. Y. 1981a. Adsorption of the defective phage PBS Z1 to Bacillus subtilis 168 Wt. —J. Gen. Virol. 52: 93–101.
Steensma, H. Y. 1981b. Effect of defective phages on the cell membrane of Bacillus subtilis and partial characterization of a phage protein involved in killing. — J. Gen. Virol. 56: 275–286.
Steensma, H. Y. and Duermeyer, W. 1979. An enzyme-linkerd immunosorbent assay (ELISA) for PBS Z1, a defective phage of Bacillus subtilis. — J. Gen. Virol. 44: 741–746.
Weidel, W., Koch, G. und Bobosch, K. 1954. Über die Rezeptorsubstanz für den Pagen T5. I. Extraktion und Reindarstellung aus E. coli B. Physikalische, chemische und funktionelle Charakterisierung. — Z. Naturforsch., B. 9: 573–579.
Zarybnicky, V., Reich, M. and Wolf, G. 1980. A mathematical model for the reversible two-step interaction between the T5 phage and its receptor in vitro. — FEMS Microbiol. Lett. 7: 29–33.
Zarybnicky, V., Brusdeilins, M. and Recih, M. 1981. Thermodynamic and kinetic studies of the interaction between T5-phages and their receptor, isolated from Escherichia coli B, in the absence and presence of Triton X-100. — Biophys. J. 35: 615–636.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Steensma, H.Y. An evaluation of the rate constants for the adsorption of the defective phage PBS Z to Bacillus subtilis . Antonie van Leeuwenhoek 48, 183–188 (1982). https://doi.org/10.1007/BF00405202
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00405202