Skip to main content
SpringerLink
Log in

Non-enzymatic glycation of elastin

  • Research Papers
  • Published:
Glycosylation & Disease

Abstract

Non-enzymatic glycation of proteins is one of the key mechanisms in the pathogenesis of diabetic complications and may be significant in the age-related changes of tissues. We investigated thein vitro glycation of human aortic α-elastin, and chose and adapted methods for evaluating the degree and kinetics of glycation. α-Elastin was prepared from thoracic aortas of young accident victims and glycated by incubating with different glucose concentrations (25, 50, 75 and 100 mmol/l) in 0.2 M phosphate buffer, pH 7.8 for 30 days, at 37°C. The degree of glycation was measured by three colorimetric methods,i.e. Nitroblue tetrazolium, 2-thiobarbituric acid and hydrazine; by aminophenyl-boronate affinity chromatography which determines Amadori products; and by a fluorescence method which determines advanced glycosylation end products. The highest degree of glycation was found on day 3 after the beginning of incubation. Fluorescence, as an index of advanced glycation, consistently increased from days 5 to 24. Investigation of the properties of glycated elastin may help in understanding the importance of this long-lived protein for the age-related changes in tissues and for diabetic complications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Cerami A. Hypothesis — glucose as a mediator of aging.J Am Ger Soc 1985:33; 624–34.

    Google Scholar 

  2. Kristal B, Bung P. An emerging hypothesis; induction of aging by free radical and Maillard reaction.J Gerontol 1992:47; B107-B111.

    Article  CAS  PubMed  Google Scholar 

  3. Kennedy L, Beynos JW. Non-enzymatic glycosylation and the chronic complications of diabetes: an overview.Diabetologia 1984:26; 93–8.

    Article  CAS  PubMed  Google Scholar 

  4. Vlassara H, Brownlee M, Cerami A. Non-enzymatic glycosylation: role in the pathogenesis of diabetic complications.Clin Chem 1986:32; B37-B41.

    CAS  PubMed  Google Scholar 

  5. Trivelli LA, Kanney HM, Lai HT. Hemoglobin components in patients with diabetes mellitus.New Engl J Med 1971:284; 353–7.

    Article  CAS  PubMed  Google Scholar 

  6. Bunn HF. Structural heterogeneity of human HbA due to nonenzymatic glycosylation.Biol Chem 1979:254; 3892–8.

    CAS  Google Scholar 

  7. McDonald NH. Glycosylated minor components of human adult hemoglobin.J Biol Chem 1978:253; 2327–37.

    CAS  PubMed  Google Scholar 

  8. Miller JA, Gravalles E, Bunn HF. Non-enzymatic glycosylation of erythrocyte membrane proteins.J Clin Invest 1980:65; 896–901.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  9. Kirstein M, Brett J, Radoff J, et al. Advanced protein glycosylation induces transendothelial human monocyte chemotaxis and secretion of platelet-dirived growth factor: role in vascular disease of diabetes and aging.Proc Natl Acad Sci USA 1990:87; 9010–4.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  10. Calvo C, Luo BY, Puygranier F, et al. Characterization and metabolism of glycated HDL in diabetic patients.Adv Exp Med Biol 1988:243; 165.

    Article  CAS  PubMed  Google Scholar 

  11. Brownless M, Vlassara H, Cerami A. Inhibition of heparin catalyzed human antithrombin III activity by nonenzymatic glycosylation: possible role in fibrin deposition in diabetes.Diabetes 1984:33; 532–5.

    Article  Google Scholar 

  12. Coradello H, Lubee G, Pollack A, Sternberg M. Enzyme activities of native non-enzymatically glycosylated trypsin, chymotrypsin and papain.Paediar Paedol 1982:17; 457–64.

    CAS  Google Scholar 

  13. Dolhofer R, Wicland OH. Preparation and biological properties of glycosylated insulin.FEBS Lett 1979:100; 133–6.

    Article  CAS  PubMed  Google Scholar 

  14. Trueb B, Fluckiger R, Winterhalter KH. Nonenzymatic glycosylation of basement membrane collagen in diabetes mellitus.Collagen Rel Res 1984:4; 239–51.

    Article  CAS  Google Scholar 

  15. Kohn RR, Schnider SL. Glycosylation of human collagen.Diabetes 1982:31 (suppl 3); 47–51.

    Article  CAS  Google Scholar 

  16. Vishwanath V, Frank KF, Elmetes, et al. Glycation of skin collagen in type I diabetes mellitus.Diabetes 1986:35; 916–921.

    Article  CAS  PubMed  Google Scholar 

  17. Schnider SL, Kohn RR. Effects of age and diabetes mellitus on the solubility and nonenzymatic glycosylation of human skin collagen.J Clin Invest 1981:67; 1630–5.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  18. Monnier VM, Kohn RR, Cerami A. Accelerated age-related browning of human collagen in diabetes mellitus.Proc Natl Acad Sci USA 1984:81; 583–7.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  19. Stevens VJ, Rouzer CA, Monnier VM, Cerami A. Diabetic cataract formation: potential role of glycosylation of lens crystalins.Proc Natl Acad Sci USA 1978:75; 2918–22.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  20. Tarsio JF, Roger LA, Furcht LT. Decreased interaction of fibronectin, type IV collagen and heparin due to nonenzymatic glycation. Implications for diabetes mellitus.Biochemistry 1987:26; 1014–20.

    Article  CAS  PubMed  Google Scholar 

  21. Charonis AS, Tsilibary EC. Structural and functional changes of laminin and type IV collagen after non-enzymatic glycation.Diabetes 1992:41; 49–51.

    Article  CAS  PubMed  Google Scholar 

  22. Greene DA. Metabolic abnormalities in diabetic peripheral nerve: relation to impaired function.Metabolism 1980:92; 118–23.

    Google Scholar 

  23. Starcher BC, Galione MJ. Purification and comparison of elastins from different animal species.Anal Biochem 1976:74; 441–7.

    Article  CAS  PubMed  Google Scholar 

  24. Partridge SM, Davis HF, Adair GS. The chemistry of connective tissues soluble proteins derived from partial hydrolysis of elastin.Biochem J 1955:61; 11–15.

    CAS  PubMed Central  PubMed  Google Scholar 

  25. Johnson RN, Metcalf PA, Baker JR. Fructosamine: a new approach to the estimation of serum glycosylprotein. An index of diabetic control.Clin Chim Acta 1982:127; 87–95.

    Article  Google Scholar 

  26. McFaraland, Cataland KF, Day JF, et al. Nonenzymatic glycosylation of serum proteins in diabetes mellitus.Diabetes 1979:28; 1011–4.

    Article  Google Scholar 

  27. Kennedy AL, Mehl TD, Merimee TJ. Non-enzymatic glycosylated serum protein: spurious elevation due to free glucose in serum.Diabetes 1980:29; 413–5.

    Article  CAS  PubMed  Google Scholar 

  28. Ma A, Naughton A, Cameron DP. Glycosylated plasma protein: a simple method for the elimination of interference by glucose in its estimation.Clin Chim Acta 1981:115; 111–7.

    Article  CAS  PubMed  Google Scholar 

  29. Kobayashi K, Yoshimoto K, Hirauchi K, Uchida K. Novel determination of glycated proteins in biological samples.Clin Chem 1992:38; 1999.

    Google Scholar 

  30. Mallia AK, Hermanson GT, Krohn RL, et al. Preparation and use of a boronic acid affinity support for separation and quantitation of glycosylated hemoglobin.Analyt Lett. 1981:14; 649.

    Article  CAS  Google Scholar 

  31. Sedmak JJ, Crossberg SE. A rapid sensitive and versatile assay for protein using Coomassie Brilliant Blue G250.Anal Biochem 1977:79; 544–52.

    Article  CAS  PubMed  Google Scholar 

  32. Fu MX, Knecht KJ, Thorpe SR, Baynes JW. Role of oxygen in cross-linking and chemical modification of collagen by glucose.Diabetes 1992:41; 42–8.

    Article  CAS  PubMed  Google Scholar 

  33. Bitensky MW, Kowluru A, Kowluru RA. Nonenzymatic glycation and protein recognition. In: Baynes JW, Monnier VM, eds.The Maillard Reaction in Aging, Diabetes and Nutrition. New York: Alan R Liss, 1989; 185–204.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology/Immunology, University School of Medicine, 5800, Pleven, Bulgaria

    Steffan Baydanoff & Emiliana Konova

  2. Central Clinical Laboratory, University School of Medicine, 5800, Pleven, Bulgaria

    Ivanka Dosheva

  3. Department of Forensic Medicine, University School of Medicine, 5800, Pleven, Bulgaria

    Plamen Dorovski

Authors
  1. Steffan Baydanoff
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Emiliana Konova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ivanka Dosheva
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Plamen Dorovski
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Baydanoff, S., Konova, E., Dosheva, I. et al. Non-enzymatic glycation of elastin. Glycosylation & Disease 1, 53–58 (1994). https://doi.org/10.1007/BF00917469

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00917469

Keywords

Search

Navigation