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Cardenolide content, emetic potency, and thin-layer chromatography profiles of monarch butterflies,Danaus plexippus, and their larval host-plant milkweed,Asclepias humistrata, in Florida

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Summary

This paper is the fourth in a series on cardenolide fingerprints of monarch butterflies (Danaus plexippus, Danainae) and their host-plant milkweeds (Asclepiadaceae) in the eastern United States. Cardenolide concentrations ofAsclepias humistrata plants from north central Florida ranged from 71 to 710 µg/0.1 g dry weight, with a mean of 417 µg/0.1 g. Monarchs reared individually on these plants contained cardenolide concentrations ranging from 243 to 575 µg/0.1 g dry weight, with a mean of 385 µg/0.1 g. Cardenolide uptake by butterflies was independent of plant concentration, suggesting that sequestration saturation occurs in monarchs fed cardenolide-rich host plants. Thinlayer chromatography resolved 19 cardenolides in the plants and 15 in the butterflies. In addition to humistratin,A. humistrata plants contained several relatively non-polar cardenolides of the calotropagenin series which are metabolized to more polar derivatives in the butterflies. These produced a butterfly cardenolide fingerprint clearly distinct from those previously established for monarchs reared on otherAsclepias species. In emetic assays with the blue jay,Cyanocitta cristata, the 50% emetic dose (ED50) per jay was 57.1 µg, and the average number of ED50 units per butterfly was 13.8, establishing that this important south eastern milkweed produces highly emetic, chemically defended monarchs. Our data provide further support for the use of cardenolide fingerprints of wild-caught monarchs to make ecological predictions concerning defence against natural enemies, seasonal movement and larval host-plant utilization by monarch butterflies during their annual cycle of migration, breeding and overwintering.

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Author notes

  1. Stephen B. Malcolm

    Present address: Department of Biological Sciences, Western Michigan University, 49008, Kalamazoo, Michigan, USA

Authors and Affiliations

  1. Department of Chemistry, Louisiana State University, 71115, Shreveport, Louisiana, USA

    Ronald A. Martin

  2. Department of Biological Sciences, Louisiana State University, 71115, Shreveport, Louisiana, USA

    Steven P. Lynch

  3. Department of Zoology, University of Florida, 32611, Gainesville, Florida, USA

    Lincoln P. Brower & Stephen B. Malcolm

  4. Department of Biology, Imperial College, Silwood Park, SL5 7PY, Ascot, Berks, United Kingdom

    Stephen B. Malcolm

  5. Department of Entomology and Nematology, University of Florida, 32611, Gainesville, Florida, USA

    Tonya Van Hook

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  1. Ronald A. Martin
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  3. Lincoln P. Brower
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Martin, R.A., Lynch, S.P., Brower, L.P. et al. Cardenolide content, emetic potency, and thin-layer chromatography profiles of monarch butterflies,Danaus plexippus, and their larval host-plant milkweed,Asclepias humistrata, in Florida. Chemoecology 3, 1–13 (1992). https://doi.org/10.1007/BF01261450

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