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Improved method for extraction of mycobacterial DNA from blood

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Conclusions

In attempting to improve the sensitivity of MAV PCR for blood, we have evaluated a new DNA extraction method exploiting the high resistance of mycobacteria to chemical and physical agents. Our experiments indicate that pretreatment of the blood sample using proteolysis and a detergent partially eliminates contaminating human DNA (up to 40%) and may contribute to removing inhibitors. Although the method produces a crude DNA preparation, inclusion of a chloroform extraction step combined with the above mentioned pretreatment makes further purification unnecessary.

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Authors and Affiliations

  1. Institut für Mikrobiologie und Immunologie, Krankenhaus Zehlendorf-Heckeshorn, Zum Heckeshorn 33, D-14109, Berlin, Germany

    D. Neugebauer, H. Mauch & A. Roth

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  1. D. Neugebauer
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  2. H. Mauch
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  3. A. Roth
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Neugebauer, D., Mauch, H. & Roth, A. Improved method for extraction of mycobacterial DNA from blood. Experientia 52, 302–303 (1996). https://doi.org/10.1007/BF01919519

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  • DOI: https://doi.org/10.1007/BF01919519

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